| 仔猪李斯特杆菌的分离与鉴定 |
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| 引用本文： | 李浩欣,段纲,项勋,祖菲,杨志远,朱旗,代飞燕,常华.仔猪李斯特杆菌的分离与鉴定[J].中国畜牧兽医,2015,42(7):1883-1889. |
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| 作者姓名： | 李浩欣 段纲 项勋 祖菲 杨志远 朱旗 代飞燕 常华 |
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| 作者单位： | 云南农业大学动物科学技术学院, 昆明 650201 |
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| 基金项目： | 云南省林业厅野生禽类重大动物疫病监测项目(K2400033);云南省高端人才引进计划项目(A3006886) |
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| 摘 要： | 为了对某猪场病死仔猪的死亡病因进行调查,本试验采集病料进行研究,采用鉴别培养基分离、生化特性鉴定、PCR扩增细菌16S rRNA基因及测序分析等方法对样品中存在的细菌进行分离鉴定,结果显示分离菌为李斯特杆菌。采用标准K-B纸片法对分离菌株进行20种抗菌药物的药敏试验,并对其生长曲线进行测定。结果显示,此菌株对青霉素G、磷霉素、阿莫西林等药物高敏,而对呋喃唑酮等药物耐药。37 ℃培养6~14 h后细菌处于增殖期。本研究为中国仔猪李斯特杆菌病细菌学检测及治疗提供了科学依据。
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| 关 键 词： | 仔猪 李斯特杆菌 鉴定 16S rRNA |
| 收稿时间： | 2014-11-27 |
Isolation and Identification of Listeria in Piglets |
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| LI Hao-xin,DUAN Gang,XIANG Xun,ZU Fei,YANG Zhi-yuan,ZHU Qi,DAI Fei-yan,CHANG Hua.Isolation and Identification of Listeria in Piglets[J].China Animal Husbandry & Veterinary Medicine,2015,42(7):1883-1889. |
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| Authors: | LI Hao-xin DUAN Gang XIANG Xun ZU Fei YANG Zhi-yuan ZHU Qi DAI Fei-yan CHANG Hua |
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| Institution: | College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | The assay was aimed to investigate the cause of death of diseased and dead piglets,we collected samples and identified by growth characteristics of bacteria in different mediums,biochemistry identification,PCR amplification of 16S rRNA genes and sequence analysis.The results showed that the isolate was Listeria.Drug sensitive test of the isolate to 20 antibiotics was detected.The results showed that this bacterium was highly sensitive to penicillin G,fosfomycin and amoxicillin and so on;However,it was resistant to furazolidone and so on.The growth curve of Listeria showed that the bacterium was in the proliferation period when 37 ℃ cultured 6 to 14 h.The results of this study provided scientific bases for detection and treatment of listeriosis in China. |
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| Keywords: | piglets Listeria identification 16S rRNA |
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