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| 绵羊骨形态发生蛋白4(BMP4)的生物信息学分析 | |
| 引用本文： | 樊振华,张秋月,范瑞文,任玉红.绵羊骨形态发生蛋白4(BMP4)的生物信息学分析[J].中国畜牧兽医,2015,42(6):1370-1376. |
| 作者姓名： | 樊振华 张秋月 范瑞文 任玉红 |
| 作者单位： | 1. 山西省农业科学研究院畜牧兽医研究所, 太原 030032; 2. 山西农业大学动物科技学院, 太谷 030801 |
| 基金项目： | 山西省科技攻关项目(20140311019-3) |
| 摘 要： | 为探究绵羊骨形态发生蛋白4(bone morphogenetic protein 4,BMP4)的生物学特性,本试验综合运用NCBI、DNAMAN、DNAStar、TMHMM Server v.2.0、PsortⅡ、SignalP等多种生物信息学软件推测绵羊BMP4基因序列性质及编码蛋白的理化性质、疏水性、磷酸化位点、保守性结构域和二级结构,并用SWISS-MODEL Workspace预测三维结构.结果显示,绵羊BMP4基因与各物种的BMP4基因存在很高的同源性,编码蛋白是一个不稳定的亲水蛋白,不存在跨膜区,很可能位于细胞核中,且存在信号肽.BMP4具有18个磷酸化位点,通过功能域的预测发现,含有2个功能域,即TGF-β超家族和TGF-β前肽超家族.这与BMP4基因家族的功能相一致,证明了绵羊BMP4是一种生长因子,具有信号传导功能.蛋白三维结构预测结果与模板3bmp.1.A同源性达到了88.29%.绵羊BMP4基因的生物信息学分析可为以后实践中深入研究其功能提供参考. |
| 关 键 词： | 绵羊 骨形态发生蛋白4(BMP4) 功能预测 生物信息学 |
| 收稿时间： | 2014-11-25 |
Bioinformatics Analysis of Bone Morphogenetic Protein 4 (BMP4) of Sheep |
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| FAN Zhen-hua,ZHANG Qiu-yue,FAN Rui-wen,REN Yu-hong.Bioinformatics Analysis of Bone Morphogenetic Protein 4 (BMP4) of Sheep[J].China Animal Husbandry & Veterinary Medicine,2015,42(6):1370-1376. | |
| Authors: | FAN Zhen-hua ZHANG Qiu-yue FAN Rui-wen REN Yu-hong |
| Institution: | 1. Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China; 2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China |
| Abstract: | The assay was aimed to explore the biological characteristics of bone morphogenetic protein 4 (BMP4) of sheep,NCBI,DNAMAN DNAStar,TMHMM Server v.2.0,PsortⅡ,SignalP various bioinformatical softwares were used to speculate the physical and chemical properties,hydrophobic property,phosphorylation site,conservative structure domain,protein secondary structure of BMP4 protein.Also,the three-dimensional structure was forecasted with the SWISS-MODEL Workspace software.The results indicated that the BMP4 of sheep had high homologies with the BMP4 of various species.The encoded protein was a hydrophilic protein which was unstable.There was no transmembrane regions and it was likely to be located in the nucleus.What was more,there was signal peptide and eighteen phosphorylation sites.Through the forecast of functional domains,the protein had two functional domains,including the transforming growth factor-beta (TGF-beta) superfamily and TGF-beta propeptide superfamily.The result was consistent with the function of BMP4 gene family,it also demonstrated that BMP4 was a growth factor and it had the function of signal transduction.The amino acid homology between the predicted 3D structure of protein and template 3bmp.1.A was 88.29%.The bioinformatics analysis of BMP4 gene could provide reference for the further study in practice. |
| Keywords: | sheep bone morphogenetic protein 4 (BMP4) functional prediction bioinformatics |
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