| 不同检测材料对ELISA检测禽白血病病毒-p27抗原结果的影响 |
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| 引用本文： | 沈学怀,张丹俊,潘孝成,赵瑞宏,胡晓苗,戴银,侯红艳,朱传民.不同检测材料对ELISA检测禽白血病病毒-p27抗原结果的影响[J].中国畜牧兽医,2015,42(5):1294-1300. |
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| 作者姓名： | 沈学怀 张丹俊 潘孝成 赵瑞宏 胡晓苗 戴银 侯红艳 朱传民 |
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| 作者单位： | 安徽省农业科学院畜牧兽医研究所, 合肥 230031 |
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| 基金项目： | 国家现代农业产业技术体系项目(CARS-41);安徽省家禽产业技术体系专项;安徽省农科院科技创新团队项目(11C0404);国家自然科学基金"MHCⅠ分子靶向性疫苗载体的构建及其免疫作用的机理"(31302044) |
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| 摘 要： | 为探讨不同检测材料对ELISA检测禽白血病病毒(ALV)-p27抗原结果的影响,试验首先对180日龄地方品种母鸡的泄殖腔棉拭子ALV-p27抗原进行ELISA检测,选取部分阳性鸡和阴性鸡进行分组试验,采用ELISA对试验鸡血清、蛋清和病毒分离的细胞培养液进行ALV-p27抗原检测,采用RT-PCR方法检测血液ALV特异性基因。结果显示,阳性组中以血清、蛋清和细胞培养液作为ELISA ALV-p27抗原检测材料,其检测阳性率均低于泄殖腔棉拭子,血清检测的阳性个体包含全部蛋清和细胞培养液ELISA检测的阳性个体。ELISA检测数据的相关性分析显示,只有血清和细胞培养液检测数据间存在显著性相关,线性关系方程为Y(细胞上清)=1.8439X(血清)-0.1469,R2=0.937;阳性组中ALV-p27基因检测阳性率低于泄殖腔棉拭子,但高于血清、蛋清和细胞培养液,其包含所有血清ELISA检测的阳性样品;外源性ALV-J gp85基因阳性率仅为29.17%,且阳性样品均属于血清ELISA阳性样品。综上所述,成年鸡以泄殖腔棉拭子作为ELISA ALV-p27抗原检测材料存在假阳性结果,蛋清和细胞培养液作为检测材料存在漏检的可能,血清作为ELISA检测材能够更准确地反映成年鸡群ALV感染状态。
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| 关 键 词： | 禽白血病病毒 ALV-p27抗原 ELISA 检测材料 |
| 收稿时间： | 2014-09-10 |
Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA |
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| SHEN Xue-huai,ZHANG Dan-jun,PAN Xiao-cheng,ZHAO Rui-hong,HU Xiao-miao,DAI Yin,HOU Hong-yan,ZHU Chuan-min.Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA[J].China Animal Husbandry & Veterinary Medicine,2015,42(5):1294-1300. |
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| Authors: | SHEN Xue-huai ZHANG Dan-jun PAN Xiao-cheng ZHAO Rui-hong HU Xiao-miao DAI Yin HOU Hong-yan ZHU Chuan-min |
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| Institution: | Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China |
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| Abstract: | In order to study the effect of different test materials on the detected results of avian leukosis virus (ALV)-p27 antigen by ELISA, the cloacal swabs ALV-p27 antigen were detected by ELISA in 180-day local breeds hen, and the parts of the positive and negative chicken was selected to group test, the serum, egg whites and cell supernatant ALV-p27 antigen were detected by ELISA, the specific genes of ALV were detected by RT-PCR in blood.The results showed that serum, egg white and cell supernatant as ELISA test materials, the positive rate lower of than cloacal swabs, and serum ALV-p27 positive samples include all egg whites and cell supernatant positive samples in positive group.It was a significant correlation between ELISAs with serum and cell supernatant (linear equation:Y=1.8439X-0.1469, the correl was 0.937).In positive group, ALV-p27 gene positive rate lower than cloacal swabs ELISA, but higher than the serum, egg and cell culture medium, and ALV-p27 gene positive samples include serum positive samples by ELISA.ALV-J gp85 gene positive rate of 29.17%, and all positive samples were included in the serum ALV-p27 positive samples.The results suggested that the cloacal swabs as test material may occur false positive results, and egg whites and cell supernatant may occur undetected by ELISA ALV-p27 antigen assay in adult chicken, serum as test material in ELISA could more accurately reflect the state of adult chickens infected with ALV. |
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| Keywords: | avian leukosis virus ALV-p27 antigen ELISA test materials |
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