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| 毛皮动物大肠杆菌对氟喹诺酮类药物及中药的耐药性研究 | |
| 引用本文： | 于杰,曲海娜,张灿,李文立,任慧英.毛皮动物大肠杆菌对氟喹诺酮类药物及中药的耐药性研究[J].中国畜牧兽医,2015,42(6):1597-1601. |
| 作者姓名： | 于杰 曲海娜 张灿 李文立 任慧英 |
| 作者单位： | 1. 青岛农业大学动物科技学院, 青岛 266109; 2. 山东省平度市职业中等专业学校, 平度 266700 |
| 基金项目： | 山东省现代农业产业技术体系毛皮动物产业创新团队(SDAIT-18-011-04) |
| 摘 要： | 本试验旨在了解毛皮动物源大肠杆菌的耐药性,有效控制毛皮动物细菌病的发生.针对14株分离自毛皮动物的大肠杆菌,采用PCR方法检测氟喹诺酮类耐药基因gyrA的携带情况,并对扩增得到的gyrA基因进行克隆测序和同源性分析;进一步针对gyrA基因制备地高辛标记的核酸探针,使用斑点杂交检测大肠杆菌对氟喹诺酮类药物的耐药情况;使用牛津杯法检测了大肠杆菌分离株对复方中药的敏感性.结果显示,PCR检出gyrA基因的阳性携带率为57.1%,斑点杂交检出耐氟喹诺酮类大肠杆菌的阳性率为50.0%,对复方中药的耐药率为28.6%.结果表明,毛皮动物对氟喹诺酮类药物的耐药性非常严重,对中药表现较高的敏感性,研究结果将为临床毛皮动物大肠杆菌病的防制提供依据. |
| 关 键 词： | 毛皮动物 大肠杆菌 氟喹诺酮类 复方中药 耐药性 |
Resistance of E.coli from Fur-bearing Animals to Fluoroquinolones and Compound Chinese Medicine |
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| YU Jie,QU Hai-na,ZHANG Can,LI Wen-li,REN Hui-ying.Resistance of E.coli from Fur-bearing Animals to Fluoroquinolones and Compound Chinese Medicine[J].China Animal Husbandry & Veterinary Medicine,2015,42(6):1597-1601. | |
| Authors: | YU Jie QU Hai-na ZHANG Can LI Wen-li REN Hui-ying |
| Institution: | 1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China; 2. Pingdu Occupation Secondary Specialized School, Pingdu 266700, China |
| Abstract: | The aim of this study was to investigate the resistance to fluoroquinolones and compound Chinese medicine, and control colibacillosis in fur-bearing animals.14 E.coli strains isolated from diseased fur-bearing animals were detected by PCR for the fluoroquinolone-resistant gyrA gene,and then the PCR fragment of gyrA gene was cloned,sequenced and analyzed for homology.Afterwards,the fragment was labeled with digoxigenin as DNA probe for dot-blot hybridization detection.The resistance to compound Chinese medicine was detected by Oxford-cup tests.The results demonstrated that the positive rates of PCR and dot-blot hybridization were 57.1% and 50.0%,respectively.Only 28.6% of the E.coli strains were resistant to compound Chinese medicine.The results indicated that the resistance to fluoroquinolones in E.coli strains from fur-bearing animals was severe,however,the isolates were much more sensitive to compound Chinese medicine.The results would provide basis for control of colibacillosis of fur-bearing animals. |
| Keywords: | fur-bearing animals E coli fluoroquinolone compound Chinese medicine antibioic resistance |
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