| 一株好氧反硝化细菌的分离与鉴定 |
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| 引用本文： | 郑建龙,时书宁,雍燕,方炳虎.一株好氧反硝化细菌的分离与鉴定[J].中国畜牧兽医,2015,42(7):1877-1882. |
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| 作者姓名： | 郑建龙 时书宁 雍燕 方炳虎 |
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| 作者单位： | 1. 华南农业大学兽医学院, 广州 510642;2. 广东大华农动物保健品股份有限公司, 云浮 527400 |
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| 基金项目： | 国家自然科学基金(31172359) |
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| 摘 要： | 本试验旨在从环境样品筛选出一株具有去除氨氮和亚硝酸盐氮的好氧反硝化细菌。通过对采集水样进行富集驯化,利用溴百里酚蓝(BTB)选择性培养基进行初筛,并以去除氨氮、亚硝酸盐氮能力为指标进行复筛,得到一株好氧反硝化细菌DG-3。在好氧条件下测定了菌株DG-3的反硝化能力,并对其进行了形态学观察、生理生化试验和16S rDNA基因序列的分析。菌株DG-3在好氧反硝化培养过程中对硝酸盐氮(138.63 mg/L)的去除率达75.57%,在整个培养过程中,仅出现了少量的亚硝酸盐氮累积,其最大累积值仅为2.06 mg/L。反硝化培养基培养及扫描电镜观察结果显示,菌株DG-3为杆状,无鞭毛,大小为(1.7~2.8)μm×(0.6~0.7)μm,菌落呈淡黄色。结合形态学、生理生化特性及16S rDNA同源性比对分析,初步判断菌株DG-3为门多萨假单胞菌(Pseudomonas mendocina)。菌株DG-3具有好氧反硝化能力,且能去除氨氮和亚硝酸盐氮,在水产养殖含氮废水的处理中具广阔的应用前景。
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| 关 键 词： | 好氧反硝化 硝酸盐 氨氮 鉴定 |
| 收稿时间： | 2015-01-20 |
Isolation and Identification of an Aerobic Denitrifying Bacterium |
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| ZHENG Jian-long,SHI Shu-ning,YONG Yan,FANG Bing-hu.Isolation and Identification of an Aerobic Denitrifying Bacterium[J].China Animal Husbandry & Veterinary Medicine,2015,42(7):1877-1882. |
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| Authors: | ZHENG Jian-long SHI Shu-ning YONG Yan FANG Bing-hu |
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| Institution: | 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Guangdong Dahuanong Animal Health Products Co., Ltd., Yunfu 527400, China |
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| Abstract: | The present study was aimed to isolate an aerobic denitrifying bacterium from environmental samples.The collecting water samples were enriched and domesticated,the bromothymol blue (BTB) selective medium were used for screening.Based on the aerobic denitrification activity,ammonium and nitrite nitrogen removing ability,the strain DG-3 was isolated from the sample.The strain was identified according to its aerobic denitrification ability,morphological,physiological and biochemical properties and phylogenetic analysis of its 16S rDNA sequence.The results showed that the reduction efficiency of strain DG-3 was up to 75.57% under aerobic conditions with initial NO3--N concentration of 138.63 mg/L,and the accumulated nitrite appeared during the process of denitrification,the max concentration was 2.06 mg/L.The morphology of strain DG-3 was with an average size of (1.7 to 2.8)μm×(0.6 to 0.7)μm and a pale yellow surface.DG-3 was identified as Pseudomonas mendocina by morphological and biochemical characteristics and molecular properties.It could be concluded that DG-3 had an aerobic denitrification ability with removing ammonium and nitrite,which showed a great potential in nitrogen wastewater treatment. |
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| Keywords: | aerobic denitrification nitrate ammonium identification |
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