首页 | 本学科首页   官方微博 | 高级检索  
     检索      

京星黄鸡白细胞介素-2基因与H/L的关联分析
引用本文:赵会静,张敬敬,文杰,刘冉冉,郑麦青,李庆贺,赵桂苹.京星黄鸡白细胞介素-2基因与H/L的关联分析[J].中国畜牧兽医,2015,42(8):2098-2103.
作者姓名:赵会静  张敬敬  文杰  刘冉冉  郑麦青  李庆贺  赵桂苹
作者单位:中国农业科学院北京畜牧兽医研究所, 北京 100193
基金项目:基本科研业务费(2012cj-5);中国农业科学院创新工程(ASTIP-IAS04)
摘    要:本研究选用鸡白细胞介素2(interleukin-2,IL-2)基因作为影响鸡免疫性状的候选基因。以京星黄鸡为研究对象,采用直接测序方法对IL-2基因的5个SNPs位点的基因型频率和等位基因频率、单倍型及其对异噬性粒细胞/淋巴细胞(H/L)指标的影响进行了研究。结果表明,所有位点在试验群体中都处于Hardy-Weinberg平衡状态,5个SNPs位点的多态性都较低,只检测到两种基因型,且纯合基因型为优势等位基因。连锁不平衡分析表明SNP1和SNP2处于完美连锁状态,SNP3、SNP4和SNP5也处于完美连锁状态。单倍型分析结果表明,京星黄鸡试验群体中共检测到4种单倍型,其中单倍型Hap1 (TGACT)为优势单倍型。最小二乘分析结果表明,这5个SNPs位点不同基因型及单倍型组合对H/L的影响没有显著差异(P> 0.05)。这些结果提示以后的研究需要关注IL-2基因其他位置的SNP位点以及与更多免疫指标之间的关系,以更深入地了解IL-2在鸡免疫系统中的作用。

关 键 词:  IL-2基因  异噬性粒细胞/淋巴细胞  关联分析  
收稿时间:2015-01-12

The Association Analysis between IL-2 Gene and H/L in Jingxinghuang Chicken
ZHAO Hui-jing,ZHANG Jing-jing,WEN Jie,LIU Ran-ran,ZHENG Mai-qing,LI Qing-he,ZHAO Gui-ping.The Association Analysis between IL-2 Gene and H/L in Jingxinghuang Chicken[J].China Animal Husbandry & Veterinary Medicine,2015,42(8):2098-2103.
Authors:ZHAO Hui-jing  ZHANG Jing-jing  WEN Jie  LIU Ran-ran  ZHENG Mai-qing  LI Qing-he  ZHAO Gui-ping
Institution:Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Abstract:Interleukin-2(IL-2) gene was regarded as a candidate gene for immune function of Jingxinghuang chicken in this study.The genotype frequencies, allele frequencies, haplotypes and association with H/L of five SNPs were studied by direct sequencing.The results indicated that all the five loci fitted Hardy-Weinberg equilibrium and showed low degrees of heterozygosity with two genotypes detected.Meanwhile, the homozygosis genotype was predominant in all the five loci.Analysis of linkage disequilibrium (LD) suggested that SNP1 and SNP2 showed perfect LD, whereas SNP3, SNP4 and SNP5 showed perfect LD.Analysis of haplotypes revealed four haplotypes, among which Hap1 (TGACT) was predominant.The least square method (LSM) analysis showed that there were no significant difference between different genotypes and haplotype combinations(P> 0.05).The results suggested that more SNPs of IL-2 gene and association with additional immune traits would be investigated in future studies for better understand the roles in immune systems that chicken IL-2 playing.
Keywords:chicken  IL-2 gene  heterophil/lymphocyte  association analysis  
本文献已被 CNKI 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号