| 一株H5N6亚型禽流感病毒对鸭和小鼠的致病性研究 |
| |
| 引用本文： | 刘永法,黄玉梅,张宗尧,万红,王媚,吕嘉敏,罗开健.一株H5N6亚型禽流感病毒对鸭和小鼠的致病性研究[J].中国畜牧兽医,2015,42(8):2176-2182. |
| |
| 作者姓名： | 刘永法 黄玉梅 张宗尧 万红 王媚 吕嘉敏 罗开健 |
| |
| 作者单位： | 华南农业大学兽医学院, 广州 510642 |
| |
| 基金项目： | 广东省科技计划项目(2012A020100001) |
| |
| 摘 要： | 为了研究一株从鹭中分离到的禽流感病毒(AIV)A/Heron/Guangdong/C1/2013(H5N6)对鸭和小鼠的致病力,本研究通过对鸭和小鼠滴鼻点眼和鸡的颈静脉注射进行攻毒试验,观察其致病力和组织病理学等变化,对其生物学特性进行初步研究。结果显示,该毒株的鸡胚半数感染量(EID50)为10-8.16/0.1 mL,静脉接种致病指数(IVPI)为2.76。对鸭的半数致死量(LD50)为10-4.0/0.2 mL,对小鼠的LD50为10-4.67/0.05 mL。以106 EID50/只滴鼻点眼感染鸭,主要表现为食欲下降、精神萎靡、肿头流泪等症状,大多数鸭在感染后4~7 d死亡,感染后第7 天肝脏、肺脏、肾脏仍在排毒,解剖可见心包积液、肺脏淤血、肾脏肿大等症状,病理切片可见心脏、肝脏、脾脏、肾脏炎性细胞浸润,脑细胞核固缩等病变。以5×105 EID50/只滴鼻感染小鼠,主要表现为食欲下降、精神萎靡、被毛粗乱、聚堆等症状,大部分小鼠在感染后5~7 d死亡,第7天时只有肺脏仍在排毒,各脏器解剖学病变不明显,病理切片可见心脏、肾脏、肺脏炎性细胞浸润,脑细胞核固缩等病变。研究结果表明,该H5N6亚型AIV毒株对鸭和小鼠具有很强的致病力,IVPI大于1.2,为高致病性AIV,本研究为H5N6亚型AIV研究和防控提供了理论基础。
|
| 关 键 词： | H5N6亚型禽流感病毒 鸭 小鼠 |
| 收稿时间： | 2015-01-14 |
Pathogenicity of a Strain of H5N6 Subtype Avian Influenza Virus in Ducks and Mice |
| |
| LIU Yong-fa,HUANG Yu-mei,ZHANG Zong-yao,WAN Hong,WANG Mei,LV Jia-min,LUO Kai-jian.Pathogenicity of a Strain of H5N6 Subtype Avian Influenza Virus in Ducks and Mice[J].China Animal Husbandry & Veterinary Medicine,2015,42(8):2176-2182. |
| |
| Authors: | LIU Yong-fa HUANG Yu-mei ZHANG Zong-yao WAN Hong WANG Mei LV Jia-min LUO Kai-jian |
| |
| Institution: | College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
| |
| Abstract: | To investigate the pathogenicity of A/Hero/Guangdong/C1/2013(H5N6), an AIV strain isolated from Heron on ducks and mice, pathogenicity of this new virus strain and changing of histopathology as well as a preliminary study on its biological characteristics were studied by virus challenges test via intranasal and eye-drop and in chicken via jugular vein injection.Results showed that the EID50 of this strain of virus was 10-8.16/0.1 mL in embryonated chicken eggs and the intravenous inoculation of pathogenic index (IVPI) was 2.76.In ducks and mice, the 50% lethal doses (LD50) of it were determined to be 10-4.0/0.2 mL and 10-4.67/0.05 mL, respectively.Symptoms of infection included loss of appetite, depression, swollen head and tears after being infected with 106 EID50 per duck by intranasal and eye-drop administration.Most of ducks died 4 to 7 days post-infection, liver, lung and kidney still eliminated toxicants at day 7 post-infection.Anatomy showed symptoms of pericardial effusion, pulmonary congestion and kidney enlargement, while pathological section showed pathological change like karyopycnosis and inflammatory cell infiltration in heart, liver, kidney and spleen.Mice developed symptoms of infection like loss of appetite, depression, shaggy coat and ruffled coat after being infected with 5×105 EID50 per mouse by intranasal and eye-drop administration.Most of the infected mice died 5 to 7 days post-infection and only liver still eliminated toxicants at day 7 post-infection.Although organ anatomy showed no obvious pathological changes, pathological section showed pathological changes like karyopycnosis and inflammatory cell infiltration in heart, kidney and lung.Our research demonstrated that this H5N6 subtype AIV had a strong pathogenicity and could be defined as a highly pathogenic AIV strain as its IVPI was greater than 1.2.Our work laid a theory foundation for study, prevention and control of H5N6 subtype AIV. |
| |
| Keywords: | H5N6 subtype avian influenza virus duck mouse |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
