| 混池法鉴定小豆F2群体 |
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| 引用本文： | 王均帅,赵璞,万平,杨凯,赵波,李奕松.混池法鉴定小豆F2群体[J].北京农学院学报,2018,33(2):23-26. |
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| 作者姓名： | 王均帅 赵璞 万平 杨凯 赵波 李奕松 |
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| 作者单位： | 北京农学院植物科学技术学院,北京,102206;农业部华北都市农业重点实验室,北京农学院生物科学与工程学院,北京102206 |
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| 基金项目： | 农业部农作物种质资源保护与利用专项项目资助(2016NWB036-28 |
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| 摘 要： | 【目的】为提高小豆遗传育种选择效率提供新方法。【方法】将9个杂交组合的35个F_2群体分别构建混池利用SSR分子标记进行真实性鉴定,并以表型性状分离作为佐证。【结果】从35个群体中鉴定出10个真杂种群体,其中17FMY2007-7群体出现叶形分离,佐证了SSR分子标记的可靠性。【结论】混池法可以有效鉴定F_2群体真实性、降低工作量、节约成本,为作图群体及其后代真实性鉴定提供新方法,以提高遗传育种选择效率。
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| 关 键 词： | 小豆 混池法 真实性鉴定 SSR标记 |
Identification of F2 population by mixing-pool in adzuki bean |
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| WANG Junshuai,ZHAO Pu,WAN Ping,YANG Kai,ZHAO Bo,LI Yisong.Identification of F2 population by mixing-pool in adzuki bean[J].Journal of Beijing Agricultural College,2018,33(2):23-26. |
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| Authors: | WANG Junshuai ZHAO Pu WAN Ping YANG Kai ZHAO Bo LI Yisong |
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| Abstract: | Objectives]To improve the selection-efficiency of genetic breeding in adzuki bean,reliability of F2 population identification was explored by mixing-pool method in adzuki bean.Methods]Each of 35 F2 populations from nine cross combinations was divided into one mixing pool respectively,and SSR marker was used for identifying true hybrid generation,and phenotypes were used as a corroborative evidence.Results]10 populations from true hybrids were identified in these 35 populations,and phenotypic segregation of 17FMY2007-7 evidenced the result of SSR marker.Conclusion]Mixing-pool method can effectively identify reliability of F2 population in adzuki bean,and improve the selection-efficiency of genetic breeding,and decrease work load and experiment cost. |
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