| 采用AFLP技术分析17个荷花(Nelumbo nucifera)品种的系统关系 |
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| 引用本文： | 衣彩洁,董爱香,李鹏飞.采用AFLP技术分析17个荷花(Nelumbo nucifera)品种的系统关系[J].北京农学院学报,2018,33(1):109-112. |
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| 作者姓名： | 衣彩洁 董爱香 李鹏飞 |
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| 作者单位： | 北京市园林科学研究院/绿化育种北京市重点实验室,100102北京;北京市莲花池公园,100055北京 |
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| 基金项目： | 北京市科委科技计划项目 |
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| 摘 要： | 【目的】采用AFLP技术,对17个荷花品种进行亲缘关系分析。【方法】从64个AFLP引物组合中筛选出10对多态性较高的引物,以17个荷花DNA为模板,进行PCR扩增,并对产物进行聚丙烯凝胶电泳分析。【结果】共获得条带523条,其中多态性条带370条,多态性比例达到70.75%;根据扩增条带信息进行聚类,可将17个品种分为2组;其中有5对引物,每对引物单独使用均可将17个品种完全区分开。【结论】AFLP用于鉴别荷花品种,具有较高的可行性;本研究为荷花分类和杂交育种的亲本选择提供参考信息。
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| 关 键 词： | 荷花 AFLP 亲缘关系 |
Analysis on genetic relationship of 17 Nelumbo nucifera cultivars using AFLP |
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| YI Caijie,DONG Aixiang,LI Pengfei.Analysis on genetic relationship of 17 Nelumbo nucifera cultivars using AFLP[J].Journal of Beijing Agricultural College,2018,33(1):109-112. |
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| Authors: | YI Caijie DONG Aixiang LI Pengfei |
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| Abstract: | Objective] AFLP (Amplified Fragments Length Polymorphism) was used to identify and classify 17 lotus cultivars (including 6 new cultivars and their parents).Methods] 10 primer combinations with high polymorphism were selected from 64 AFLP primer combinations using 17 lotus DNA as templates.Results] A total of 523 fragments were amplified,of which 370 were polymorphic fragments,and the polymorphism percentage was 70.75％.Then by cluster analysis,17 lotus cultivars were classified into 2 groups.There were 5 primer combinations,using one of which the 17 cultivars could be separated completely.Conclusion] AFLP was a reliable and efficient method to identify lotus cultivars.This study provided theoretical reference for classification of lotus and parents selection for hybridization. |
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