| 36份新疆野生黄花苜蓿SSR 水平的差异性研究 |
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| 引用本文： | 李倩,若扎·扎尔汗,李陈建,张博,王玉祥.36份新疆野生黄花苜蓿SSR 水平的差异性研究[J].中国草地学报,2021(2). |
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| 作者姓名： | 李倩 若扎·扎尔汗 李陈建 张博 王玉祥 |
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| 作者单位： | 新疆农业大学草业与环境科学学院;西部干旱区草地资源与生态教育部重点实验室 |
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| 基金项目： | 国家自然基金“新疆野生黄花苜蓿种质资源遗传特性与核心种质构建研究”(31860675);农业农村部国家牧草产业技术体系岗位科学家项目(CARS-34)资助。 |
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| 摘 要： | 以36份新疆野生黄花苜蓿为材料,采用SSR分子标记对其遗传多样性进行分析,以期为新疆黄花苜蓿资源评价和核心种质库构建提供基础数据。结果表明,9对SSR分子标记引物PCR共扩增出93条条带,每对引物扩增出的条带数变异范围为3~19条,平均10.33条/对,多态条带总数为86个,多态条带百分比为92.47%,平均多态性条带数为9.55,平均多态性比例为91.55%,表明居群间存在丰富的遗传多样性。POPGENE分析表明,在遗传相似系数0.611处可将36份野生黄花苜蓿材料划分为2个混合群体和4个特定类群,表明材料具有显著异质性。
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| 关 键 词： | 黄花苜蓿 居群 SSR 遗传多样性 |
Genetic Diversity of 36 Medicago falcata Populations in Xinjiang Based on SSR Analysis |
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| LI Qian,Ruozha·Zhaerhan,LI Chen-jian,ZHANG Bo,WANG Yu-xiang.Genetic Diversity of 36 Medicago falcata Populations in Xinjiang Based on SSR Analysis[J].Chinese Journal of Grassland,2021(2). |
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| Authors: | LI Qian Ruozha·Zhaerhan LI Chen-jian ZHANG Bo WANG Yu-xiang |
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| Institution: | (College of Grassland and Environment Sciences,Xinjiang Agricultural University,Urumqi 830052,China;Key Laboratory of Grassland Resources and Ecology of Ministry of Education,Western China,Urumqi 830052,China) |
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| Abstract: | The genetic diversity of 36 Medicago falcata accessions in Xinjiang were analyzed by SSR molecular markers,in order to provide basic data for the resource and core collection construction of Xinjiang M.falcata.The results showed that a total of 93 bands were amplified by 9 pairs of SSR molecular marker primers.The variation range of bands amplified by each pair of primers ranged from 3 to 19,with an average of 10.33 bands per pair.The total number of polymorphic bands was 86,the percentage of polymorphic bands was 92.47%,the average number of polymorphic bands was 9.55,and the ratio of polymorphic bands was 91.55%,indicating that there was abundant genetic diversity among populations.POPGENE analysis showed that 36 M.falcata accessions were divided into 2 mixed groups and 4 specific subgroups at the genetic similarity coefficient of 0.611,indicating that the accessions had significant heterogeneity. |
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| Keywords: | Medicago falcata Populations SSR Genetic diversity |
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