| 全局调控因子LaeA对蛹虫草生长发育和次生代谢的作用 |
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| 引用本文： | 汪滢,杨占山,鲍大鹏,李燕,吴莹莹,邹根.全局调控因子LaeA对蛹虫草生长发育和次生代谢的作用[J].食用菌学报,2022(1):20-26. |
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| 作者姓名： | 汪滢 杨占山 鲍大鹏 李燕 吴莹莹 邹根 |
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| 作者单位： | 上海市农业科学院食用菌研究所 |
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| 基金项目： | 上海市科技兴农重点攻关项目(2018-02-08-00-12-F01555);国家自然科学基金(31902089)。 |
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| 摘 要： | 通过克隆蛹虫草(Cordyceps militaris)野生型菌株Cm01的laeA基因(CmLaeA)、合成构巢曲霉(Aspergillus nidulans)的laeA基因(AsLaeA),构建不同过表达载体,利用农杆菌(Agrobacterium tumefaciens)介导转化野生型蛹虫草菌株LN9、Cm01,...
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| 关 键 词： | 蛹虫草 LaeA 子实体发育 虫草素 |
Effects of LaeA on Growth,Development and Secondary Metabolism of Cordyceps militaris |
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| WANG Ying,YANG Zhanshan,BAO Dapeng,LI Yan,WU Yingying,ZOU Gen.Effects of LaeA on Growth,Development and Secondary Metabolism of Cordyceps militaris[J].Acta Edulis Fungi,2022(1):20-26. |
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| Authors: | WANG Ying YANG Zhanshan BAO Dapeng LI Yan WU Yingying ZOU Gen |
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| Institution: | (Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,Shanghai 201403,China) |
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| Abstract: | The laeA genes of the wild type Cordyceps militaris Cm01(CmLaeA)and Aspergillus nidulans(AsLaeA)were cloned and synthesized,respectively,and then used to construct corresponding overexpression vectors.Using Agrobacterium tumefaciens mediated transformation,these overexpression vectors were then transferred into wild C.militaris strains Cm01 and LN9 respectively to generate four recombinant strains as follows:CmLaeALN9(transformation of LN9 with CmLaeA),CmLaeACm01(transformation of Cm01 with CmLaeA),AsLaeALN9(transformation of LN9 with AsLaeA)and AsLaeACm01(transformation of Cm01 with AsLaeA).Compared with the wild type strains,CmLaeACm01,AsLaeALN9 and AsLaeACm01 contained 1.5 times more cordycepin.CmLaeAcm01 produced 50%more conidia and started to develop fruiting bodies 5-7 d earlier than the wild type strains.The regulatory effect of LaeA varies with source and host strain. |
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| Keywords: | Cordyceps militaris LaeA fruiting body development cordycepin |
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