| 云南粳型地方稻资源抗稻瘟病鉴定评价 |
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| 引用本文： | 管娟,肖素勤,陈玲,张敦宇,张云,程在全.云南粳型地方稻资源抗稻瘟病鉴定评价[J].南方农业学报,2022,53(1):47-56. |
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| 作者姓名： | 管娟 肖素勤 陈玲 张敦宇 张云 程在全 |
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| 作者单位： | 1 云南大学资源植物研究院, 昆明 650504;2 云南省农业科学院生物技术与种质资源研究所, 昆明 650205 |
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| 基金项目： | 云南省青年拔尖人才专项;云南大学研究生科研创新基金项目;云南省颜龙安院士专家工作站项目 |
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| 摘 要： | 【目的】鉴定和筛选获得云南粳型地方稻抗稻瘟病种质资源，明确其稻瘟病抗性及抗病基因分布，为合理利用水稻抗稻瘟病种质资源及其布局提供参考。【方法】采用病圃自然鉴定与人工接种相结合的方法，对150份云南粳型地方稻资源的稻瘟病抗性进行鉴定，并利用9个已克隆基因（Pid4、Pi25、Pi35、Pi36、Pikm、Pigm、Pi37、Pi56、Pi33）和2个已定位基因（Pi27、Pi23）对供试稻种资源进行基因型检测，明确抗病基因在抗稻瘟病材料中的分布。【结果】从150份水稻材料中共鉴定出抗稻瘟病（叶瘟或穗颈瘟）材料45份，45份材料中携带Pi25、Pi23、Pikm、Pi35、Pi36、Pi33、Pi27、Pi37、Pid4、Pigm抗性基因的材料分别有34份（75.6%）、25（55.6%）、25份（55.6%）、22份（48.9%）、14份（31.1%）、14份（31.1%）、12份（26.7%）、10份（22.2%）、6份（13.3%）和5份（11.1%），未检测到含有Pi56的材料。45份抗性材料含有的抗性基因数量介于1~7，大部分含有3~5个，其中携带3个抗性基因材料13份占28.9%，携带4个抗性基因材料8份占17.8%，携带5个抗性基因材料11份占24.4%。抗病能力较强的材料黑壳谷携带6个抗病基因（Pi25、Pi35、Pi36、Pikm、Pi37和Pi33），光壳花糯携带5个抗病基因（Pi35、Pi36、Pikm、Pi37和Pi33）。【结论】研究获得45份云南粳型抗稻瘟病地方稻材料，鉴定获得2份（黑壳谷和光壳花糯）全生育期抗叶瘟和穗颈瘟材料，这2份抗性材料均携带Pi35-Pi36-PikmPi37-Pi33基因组合，可作为抗稻瘟病分子育种的优异抗源材料。
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| 关 键 词： | 水稻 稻瘟病 表型鉴定 基因分析 云南省 |
| 收稿时间： | 2022-01-10 |
Evaluation of resistance to rice blast of japonica rice landrace resources in Yunnan |
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| GUAN Juan,XIAO Su-qin,CHEN Ling,ZHANG Dun-yu,ZHANG Yun,CHENG Zai-quan.Evaluation of resistance to rice blast of japonica rice landrace resources in Yunnan[J].Journal of Southern Agriculture,2022,53(1):47-56. |
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| Authors: | GUAN Juan XIAO Su-qin CHEN Ling ZHANG Dun-yu ZHANG Yun CHENG Zai-quan |
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| Institution: | 1 School of Resource Plants, Yunnan University, Kunming 650504, China;2 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China |
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| Abstract: | 【Objective】Yunnan japonica rice blast resistant germplasm resources were obtained through identification and screening. Furthermore，the distribution of rice blast resistance genes in Yunnan japonica local rice resources were studied，which provided reference for rational utilization and distribution of rice blast resistance germplasm resources. 【Method】The blast resistance of Yunnan japonica local rice resources was identified by natural identification in nursery and artificial inoculation. Rice blast resistance of 150 Yunnan japonica local rice resources was identified. Nine cloned genes（Pid4，Pi25，Pi35，Pi36，Pikm，Pigm，Pi37，Pi56，Pi33）and two localized genes（Pi27，Pi23）were used to detect the genotypes of the tested rice germplasms to determine the distribution of resistance genes in rice blast resistance materials.【Result】A total of 45 rice materials resistant to rice blast（leaf blast or panicle neck blast）were identified from 150 rice materials. The numbers of resistant materials carrying resistance genes Pi25，Pi23，Pikm，Pi35，Pi36，Pi33，Pi27， Pi37，Pid4 and Pigm were 34（75.6%），25（55.6%），25（55.6%），22（48.9%），14（31.1%），14（31.1%），12（26.7%）， 10（22.2%），6（13.3%）and 5（11.1%）. No material containing Pi56 was detected. The number of resistance genes in 45 resistant materials ranged from 1 to 7，and most of them contained 3 to 5 resistance genes，including 13 materials carr-ying three resistance genes（28.9%），8 materials carrying 4 resistance genes（17.8%）and 11 materials carrying 5 resistance genes（24.4%）. Heikegu with strong disease resistance carried 6 resistance genes（Pi25，Pi35，Pi36，Pikm，Pi37 and Pi33），and Guangkehuanuo carried 5 resistance genes（Pi35，Pi36，Pikm，Pi37 and Pi33）.【Conclusion】In this study，45 japonica rice blast resistant materials in Yunnan were obtained. And 2 materials with resistance to leaf blast and neck blast were identified as Heikegu and Guangkehuanuo during the whole growth period they the gene combination of Pi35-Pi36-Pikm-Pi37-Pi33. This study can provide resistance source materials for molecular breeding and gene mapping of resistance to rice blast. |
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