Observations on induced breeding of the Amazonian fish tambaqui, Colossoma macropomum (Cuvier) using CPE and HCG treatments |
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| Authors: | S Chellappa M S R F Cacho F A Huntingford† MCM Beveridge |
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| Institution: | Department of Zoology, University of Glasgow, Glasgow, UK;Departamento de Oceanografia e Limnologia, Centro de Biociencias, Universidade Federal do Rio Grande do Norte, Natal/RN, Brazil;Department of Zoology, University of Glasgow, Glasgow, UK;Institute of Aquaculture, University of Stirling, Stirling, UK |
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| Abstract: | Trials on induced breeding of the native Amazonian fish tambaqui, Colossoma macropomum (Cuvier), using CPE and HCG treatments, were carried out during 1992-93 at the Fish Culture Station in Natal, Brazil. Final maturation, ovulation and spermiation of tambaqui broodstock, reared in earthen ponds, were induced by injecting heteroplastic carp pituitary gland extracts (CPE) and human chorionic gonadotrophin (HCG) separately. The female spawners received double injections and the males received a single dose. Females were tranquillized using quinaldine and their genital openings were sutured before the resolving injection was administered. Latent period between the resolving injection and ovulation varied from 6 to 11 h according to the prevailing water temperature. Milt was obtained by extrusion. Stripping and dry fertilization of the eggs produced viable larvae. Relative fecundity was estimated at 140 000 eggs kg-1. Mean fertilization and hatching rates varied from 50% to 70% and from 60% to 80%, respectively. |
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