Characterization of <Emphasis Type="Italic">Dickeya</Emphasis> strains isolated from potato and river water samples in Finland |
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| Authors: | Jaana Laurila Virpi Ahola Ari Lehtinen Tiina Joutsjoki Asko Hannukkala Anne Rahkonen Minna Pirhonen |
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| Institution: | (1) MTT Agrifood Research Finland, Plant Production Research, 31600 Jokioinen, Finland;(2) MTT Agrifood Research Finland, Biotechnology and Food Research, 31600 Jokioinen, Finland;(3) Potato Research Institute, Ruosuontie 156, 16900 Lammi, Finland;(4) Department of Applied Biology, Plant Pathology, University of Helsinki, 00014 Helsinki, Finland;(5) Present address: Boreal Plant Breeding Ltd, Myllytie 10, 31600 Jokioinen, Finland |
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| Abstract: | Plant pathogenic enterobacteria in the genera Pectobacterium and Dickeya (formerly classified as Erwinia) were isolated from diseased potato stems and tubers. The isolated bacteria were identified as P. atrosepticum, P. carotovorum and pathogens in the genus Dickeya with PCR tests. Furthermore, Dickeya strains were isolated from river water samples throughout the country. Phylogenetic analysis with 16S-23S rDNA intergenic
spacer sequences suggested that the Dickeya strains could be divided into three groups, two of which were isolated from potato samples. Phylogenetic analysis with 16S
rDNA sequences and growth at 39°C suggested that one of the groups corresponds to D. dianthicola, a quarantine pathogen in greenhouse cultivation of ornamentals, while two of the groups did not clearly resemble any of
the previously characterised Dickeya species. Field trials with the strains indicated that D. dianthicola-like strains isolated from river samples caused the highest incidence of rotting and necrosis of potato stems, but some of
the Dickeya strains isolated from potato samples also caused symptoms. The results showed that although P. atrosepticum is still the major cause of blackleg in Finland, virulent Dickeya strains were commonly present in potato stocks and rivers. This is the first report suggesting that Dickeya, originally known as a pathogen in tropical and warm climates, may cause diseases in potato in northern Europe. |
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| Keywords: | Blackleg Erwinia chrysanthemi Soft rot |
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