| 鼓槌石斛组织培养及快繁技术研究 |
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| 引用本文： | 崔 波,蒋素华,王洁琼,康莹莹,梁 芳.鼓槌石斛组织培养及快繁技术研究[J].中国农学通报,2015,31(19):111-116. |
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| 作者姓名： | 崔 波 蒋素华 王洁琼 康莹莹 梁 芳 |
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| 作者单位： | 郑州师范学院生物工程研究所,郑州师范学院生物工程研究所,河南农业大学,河南农业大学,郑州师范学院生物工程研究所 |
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| 基金项目： | 省自然科学“蝴蝶兰新品种选育及产业化关键技术研究”(14B180036),河南省科技攻关项目“蝴蝶兰分子育种”(092102110128)。 |
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| 摘 要： | 以鼓槌石斛成熟果实中的种子为外植体，研究了鼓槌石斛无菌苗增殖，壮苗生根的最佳培养基配方，并对无菌苗增殖培养条件进行了优化。研究结果表明：可通过种子直接形成无菌苗、再经无菌苗增殖、壮苗生根的途径，生成完整植株，实现鼓槌石斛种苗的规模化生产；并筛选出适宜的培养基：无菌苗增殖培养基为MS+0.5mg/LNAA+2.0mg/LBA+50g/L香蕉泥，增殖系数高达4.7，且长势良好、芽苗健壮；壮苗生根培养基为1/2MS+0.3mg/LNAA，生根苗根系长而粗壮，叶色浓绿。无菌苗增殖的优化条件：白糖浓度为30g/L，有机添加物为香蕉泥，丛植芽数量为5株/丛时增殖系数最高，幼苗长势最好。
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| 关 键 词： | 春玉米 春玉米 产量 施氮水平 响应 |
| 收稿时间： | 2015/1/15 0:00:00 |
| 修稿时间： | 2015/5/29 0:00:00 |
Research on the Tissue Culture and Rapid Propagation Technology of Dendrobiun chrysotoxum Lindl |
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| Abstract: | The seeds from mature fruit of Dendrobiun chrysotoxum Lindl were used as explants to study the suitable medium for aseptic seedlings proliferation and rooting ,and the culture condition of aseptic seedlings proliferation was optimized .The results showed that:formed directly seeds aseptic seedling, then through its proliferation and rooting to generate complete plants.Thus realizing Dendrobiun chrysotoxum seedling large-scale production;selected the suitable culture medium: the optimal medium for aseptic seedling proliferation was MS 0.5 mg/LNAA 2.0 mg/LBA 50g/LBanana puree , proliferation coefficient of 4.7 and they growed well;while the optimal medium for rooting was 1/2MS 0.3 mg/LNAA,the roots of root seedlings were long and thick,the leaves were also thick. optimization conditions of proliferation: the proliferation coefficient was the highest and plants growed the best while the suitable sugar concentration was 30 g/L,the suitable organic additive was Banana puree and the suitable clump planting bud number was 5 each other |
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| Keywords: | Dendrobiun chrysotoxum Lindl aseptic germination aseptic seedlings proliferation rapid propagation |
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