| 甜高粱茎秆汁液固定化酵母酒精发酵的研究 |
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| 引用本文： | 刘荣厚,李金霞,沈飞,孙清.甜高粱茎秆汁液固定化酵母酒精发酵的研究[J].农业工程学报,2005,21(9):137-140. |
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| 作者姓名： | 刘荣厚 李金霞 沈飞 孙清 |
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| 作者单位： | 上海交通大学农业与生物学院生物质能工程研究中心,上海,201101;沈阳农业大学工程学院,沈阳,110161 |
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| 基金项目： | 辽宁省科技攻关项目(2003224003)；欧盟资助项目(ICA4-2002-10023)；农业部资助项目(2002—14—01A) |
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| 摘 要： | 本文应用一元线性回归分析法得出了甜高粱茎秆汁液锤度和可溶性总糖含量之间的关系。以甜高粱茎秆汁液为原料，在摇床及流化床反应器上进行了固定化酵母酒精发酵的试验研究。结果表明，以沈农甜杂2号甜高粱为原料，在摇床上作营养盐配比的正交试验，试验得出对提高酒精得率影响因素的主次顺序为：(NH4)2SO4，K2HPO4，MgSO4。最优组合为：0.125% K2HPO4，0.200%(NH4)2SO4，0.050% MgSO4。加营养盐的酒精得率比不加任何营养盐的酒精得率可提高4.0%～8.0%。K2HPO4，(NH4)2SO4的配比对试验结果影响极显著，MgSO4的配比对试验结果影响显著。在流化床反应器上试验，4种较优营养盐组合酒精得率近乎相等，比不加营养盐空白试验酒精得率高。从降低成本角度来讲，选择0.125% K2HPO4，0.200%(NH4)2SO4，0.010% MgSO4作为投产试验。本文为燃料乙醇的发展提供了科学的依据。
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| 关 键 词： | 甜高粱茎秆汁液 固定化酵母 营养盐 酒精发酵 流化床生物反应器 |
| 文章编号： | 1002-6819(2005)09-0137-04 |
| 收稿时间： | 5/8/2004 12:00:00 AM |
| 修稿时间： | 2005-08-16 |
Ethanol fermentation of sweet sorghum stalk juice by immobilized yeast |
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| Liu Ronghou,Li Jinxi,Shen Fei and Sun Qing.Ethanol fermentation of sweet sorghum stalk juice by immobilized yeast[J].Transactions of the Chinese Society of Agricultural Engineering,2005,21(9):137-140. |
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| Authors: | Liu Ronghou Li Jinxi Shen Fei and Sun Qing |
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| Abstract: | The relationship between the Brix and the total soluble sugar content of sweet sorghum stalk juice was determined through one-dimensional linear regression. Meanwhile, ethanol fermentation experiments were conducted in a shaking flask and a 10 L fluidized bed bioreactor with stalk juice of SNTZ No.2 sweet sorghum variety when immobilized yeast was applied. The experimental results in the shaking flask showed that the influence (sequence) to improve ethanol yield was (NH_4)_2SO_4, MgSO_4, K_2HPO_4, and the optimum ratios of K_2HPO_4, (NH_4)_2SO_4 and MgSO_4 were 0.125%, 0.200% and 0.050%, respectively. Ethanol yields with inorganic salts were increased by 4.0%~8.0% compared to without one. K_2HPO_4 and (NH_4)_2SO_4 were greatly significant to the experiment results, and MgSO_4 ranked second. The experimental results in the fluidized bed bioreactor showed that ethanol yields were nearly equal to four kinds of ideal inorganic salts. And the ethanol yields with inorganic salts were higher than that without one. It was beneficial for ethanol production when blending ratios of K_2HPO_4, (NH_4)_2SO_4 and MgSO_4 were (0.125%), 0.200% and 0.010%, respectively from the aspect of reducing cost. The research provides a scientific basis for fuel ethanol development. |
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| Keywords: | sweet sorghum stalk juice immobilized yeast inorganic salt ethanol fermentation fluidized bed bioreactor |
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