| 一株猪圆环病毒2型的分离与分子鉴定 |
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| 引用本文： | 赵朴,赵坤,李任峰,王三虎,刘兴友,陈金山,赵恒章,郑玉姝.一株猪圆环病毒2型的分离与分子鉴定[J].河南农业科学,2011,40(7):155-157. |
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| 作者姓名： | 赵朴 赵坤 李任峰 王三虎 刘兴友 陈金山 赵恒章 郑玉姝 |
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| 作者单位： | 1. 河南科技学院动物科学学院,河南,新乡453003
2. 河南科技学院动物科学学院,河南,新乡453003;动物疫病和残留物防控河南省高校工程技术研究中心,河南,新乡453003 |
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| 摘 要： | 利用PCR技术初步检测疑似PCV2感染的猪肺脏和淋巴结中的病毒.将样品处理后接种于PK-15细胞.从感染PK-15细胞PCR扩增PCV2基因片段,克隆入pMD18-T载体,并进行序列测定和比较.序列分析结果表明,分离病毒的PCR产物与国内多株PCV2核酸同源性大于98%,说明所分离的病毒为PCV2.
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| 关 键 词： | 猪圆环病毒2型 PCR分离 鉴定 |
Isolation and Molecular Identification of A Strain of Porcine Circovirus Type 2 |
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| ZHAO Pu,ZHAO Kun,LI Ren-feng,WANG San-hu,LIU Xing-you,CHEN Jin-shan,ZHAO Heng-zhang,ZHENG Yu-shu.Isolation and Molecular Identification of A Strain of Porcine Circovirus Type 2[J].Journal of Henan Agricultural Sciences,2011,40(7):155-157. |
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| Authors: | ZHAO Pu ZHAO Kun LI Ren-feng WANG San-hu LIU Xing-you CHEN Jin-shan ZHAO Heng-zhang ZHENG Yu-shu |
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| Institution: | 1,2(1.Department of Animal Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China;2.Henan Engineering Research Center of Animal Disease and Residue Prevention and Control,Xinxiang 453003,China) |
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| Abstract: | Porcine circovirus type 2(PCV2) was detected in the lungs and lymph nodes from suspected pigs infected by PCV2.Positive tissue samples were inoculated to the PK-15 cells after treatment.The PCV2 nucleic acid fragments were amplified from the infected PK-15 cells and cloned into pMD18-T vector for sequencing.Sequences comparison showed that the homology was more than 98% between the present isolate and other previous reported strains of PCV2,which indicated that the isolated virus was PCV2. |
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| Keywords: | Porcine circovirus type 2 PCR isolation Identification |
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