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| 霍阿基亚型羊驼显性白毛调控基因外显子2的克隆与序列分析 | |
| 引用本文： | 高荣琨,李建平,曲海娥,江倩,陈伟,王莹,张巧灵.霍阿基亚型羊驼显性白毛调控基因外显子2的克隆与序列分析[J].中国畜牧兽医,2013,40(8):42-45. |
| 作者姓名： | 高荣琨 李建平 曲海娥 江倩 陈伟 王莹 张巧灵 |
| 作者单位： | 1. 山西农业大学动物科技学院, 山西太谷 030801; 2. 吉林大学动物医学学院, 吉林长春 130062; 3. 吉林农业大学动物科学技术学院, 吉林长春 130118 |
| 基金项目： | 国家自然科学基金(30800807、31072097);中国博士后科学基金(20100471261) |
| 摘 要： | 羊驼作为一种高品质毛皮经济动物,对其毛色的研究可以为提高羊驼经济价值提供理论依据。本试验应用RT-PCR技术克隆羊驼显性白位点基因(KIT)第2外显子(exon2),并与其他动物相应区域进行同源性比较,结果发现其序列与牛和山羊的同源性较高,可达到96%,其编码的氨基酸更高,达98%;而与人和家鼠的同源性较低,只有78%和57%。该研究结果为加快霍阿基亚型羊驼育种进展提供了理论依据。 |
| 关 键 词： | 霍阿基亚羊驼 毛色 显性白毛调控基因 序列分析 |
Cloning and Sequence Analysis of Huacuya Alpacas KIT Gene Exon2 |
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| GAO Rong-kun,LI Jian-ping,QU Hai-e,JIANG Qian,CHEN Wei,WANG Ying,ZHANG Qiao-ling.Cloning and Sequence Analysis of Huacuya Alpacas KIT Gene Exon2[J].China Animal Husbandry & Veterinary Medicine,2013,40(8):42-45. | |
| Authors: | GAO Rong-kun LI Jian-ping QU Hai-e JIANG Qian CHEN Wei WANG Ying ZHANG Qiao-ling |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; 2. College of Animal Medicine, Jilin University, Changchun 130062, China; 3. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China |
| Abstract: | Alpaca as a kind of high quality economic animal, its colour of the research could provide a theoretical basis for improving the alpaca economic value. KIT gene was selected to investigate the relationship of KIT gene exon2 with coat color of alpacas, and to analyze the homology to other animals by using RT-PCR.The results showed that the sequence homolog was higher than cows and goats,could be up to 96%, coded amino acids were higher, reaching 98%, while the homologies of human and mouse were lower, only 78% and 57%. The results could provide theoretical basis for accelerating the progress of Huacuya alpacas breeding. |
| Keywords: | Huacuya alpacas coat color KIT gene sequence analysis |
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