| 斑鳢基因组中微卫星分布特征及野生种群遗传结构分析 |
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| 引用本文： | 上官清,陈昆慈,刘海洋,欧密,罗青,王亚坤,徐晟云,赵建.斑鳢基因组中微卫星分布特征及野生种群遗传结构分析[J].南方水产科学,2020,16(3):47-60. |
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| 作者姓名： | 上官清 陈昆慈 刘海洋 欧密 罗青 王亚坤 徐晟云 赵建 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所/农业农村部热带亚热带水产资源利用与养殖重点实验室,广东广州 510380;上海海洋大学,上海 201306;中国水产科学研究院珠江水产研究所/农业农村部热带亚热带水产资源利用与养殖重点实验室,广东广州 510380 |
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| 基金项目： | 广州市科技计划珠江科技新星专项;现代农业产业技术体系建设专项;中国水产科学研究院基本科研业务费专项;中国水产科学研究院基本科研业务费专项;广东省海洋渔业科技与产业发展专项 |
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| 摘 要： | 斑鳢(Channa maculata)是华南地区的本土经济鱼类,也是杂交鳢的亲本之一。养殖个体逃逸可能会对野生种群产生影响,存在种质混杂的风险,亟须开展野生资源的遗传背景分析。该研究分析了斑鳢基因组中微卫星标记的分布特征,筛选获得20个多态性位点构建多重PCR体系,对广州、化州、江华、南宁、阳江和邵武6个野生群体的遗传多样性和遗传结构进行了分析。结果显示,6个野生群体各位点的等位基因(Na)为3~28、有效等位基因(Ne)为1.28~14.88、观测杂合度(Ho)为0.10~1.00、期望杂合度(He)为0.14~0.95以及多态信息含量(PIC)为0.13~0.95。UPGAM系统进化树结果显示,化州和福建种群遗传关系最近,化州和江华种群遗传关系最远。该研究结果将为斑鳢的遗传监测和亲缘关系鉴定提供技术支持,为斑鳢种质资源养护及管理提供参考。
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| 关 键 词： | 斑鳢 遗传结构 微卫星标记 野生种群 |
Characteristics of micorsatellites and genetic structure of wild Channa maculata |
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| SHANGGUAN Qing,CHEN Kunci,LIU Haiyang,OU Mi,LUO Qing,WANG Yakun,XU Shengyun,ZHAO Jian.Characteristics of micorsatellites and genetic structure of wild Channa maculata[J].South China Fisheries Science,2020,16(3):47-60. |
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| Authors: | SHANGGUAN Qing CHEN Kunci LIU Haiyang OU Mi LUO Qing WANG Yakun XU Shengyun ZHAO Jian |
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| Institution: | (Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation of Ministry of Agriculture and Rural Affairs,Guangzhou 510380,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Channa maculata is not only an indigenous economic fish in South China but also a parent species of cultured hybrid snakehead.Escape of the bred individuals may have impacts on wild populations,which makes genetic background analysis of wild resources an urgently need.This study analyzed the distribution characteristics of microsatellite markers in genome of C.maculata,and developed 20 polymorphic loci to analyze the genetic diversity and genetic structure of six wild populations named as Guangzhou,Huazhou,Jianghua,Nanning,Yangjiang and Shaowu populations.The results show that the number of alleles(Na)of the six wild populations was 3?28;the number of effective alleles(Ne)was 1.28?14.88;the observed heterozygosity(Ho)was 0.10?1.00;the expected heterozygosity(He)was 0.14?0.95;the polymorphic information content(PIC)was 0.13?0.95.The UPGAM dendrogram indicates that the genetic relationship between Huazhou and Fujian populations was the closest,but that between Huazhou and Jianghua populations was the farthest.This study provides basis for the conservation and management of germplasm resources of C.maculata. |
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| Keywords: | Channa maculata Genetic structure Microsatellite marker Wild population |
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