| 哈维弧菌vhh基因缺失株的构建及其相关生物学特性研究 |
| |
| 引用本文： | 张亚秋,邓益琴,冯娟,毛灿,胡建美,苏友禄.哈维弧菌vhh基因缺失株的构建及其相关生物学特性研究[J].南方水产科学,2020,16(2):43-53. |
| |
| 作者姓名： | 张亚秋 邓益琴 冯娟 毛灿 胡建美 苏友禄 |
| |
| 作者单位： | 上海海洋大学水产与生命学院,上海201306;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300;中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572018;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300;中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572018;上海海洋大学水产与生命学院,上海201306;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300;广东省水环境与水产品安全工程技术研究中心/仲恺农业工程学院动物科技学院,广东广州510225 |
| |
| 基金项目： | 海南省自然科学基金青年基金(319QN336);国家自然科学基金(31902415);广东省自然科学基金(2018A030310695,2019A1515011833)。 |
| |
| 摘 要： | 哈维弧菌(Vibrio harveyi)是水生动物的重要病原,为研究哈维弧菌溶血素基因vhh缺失后对其生物学特性的影响,该研究利用同源重组技术构建了V.harveyi 345的vhh基因缺失突变株,并比较了野生株和突变株的生物学特性变化。结果显示,vhh基因的缺失不影响菌株的生长、胞外蛋白酶分泌、过氧化氢(H2O2)和铜离子(Cu2+)的压力感应、铁的吸收利用、15种抗生素抗性和生物膜形成等生物学特性,但会导致菌株游动和涌动显著增强;另发现,vhh基因虽然在野生菌株内高表达,对绵羊红细胞却未表现出溶血活性。结果表明该基因负调控着菌株的运动能力。该研究为认识哈维弧菌vhh基因功能研究提供新的资料。
|
| 关 键 词： | 哈维弧菌 溶血素基因vhh 基因敲除 生物学特性 细菌运动性 |
Construction of knock-out mutant of vhh in Vibrio harveyi and its related biological characteristics analysis |
| |
| ZHANG Yaqiu,DENG Yiqin,FENG Juan,MAO Can,HU Jianmei,SU Youlu.Construction of knock-out mutant of vhh in Vibrio harveyi and its related biological characteristics analysis[J].South China Fisheries Science,2020,16(2):43-53. |
| |
| Authors: | ZHANG Yaqiu DENG Yiqin FENG Juan MAO Can HU Jianmei SU Youlu |
| |
| Institution: | (College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Development&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Tropical Fisheries Research and Development Center,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya 572018,China;College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering/Guangdong Water Environment and Aquatic Product Safety Engineering Technology Research Center,Guangzhou 510225,China) |
| |
| Abstract: | Vibrio harveyi is an important pathogen of aquatic animals.In order to study the biological characteristics of the strain after the absence of hemolysin gene vhh,the knock-out mutant of vhh in V.harveyi was constructed by homologous recombination technique.Moreover,the biological characteristics of the wild type strain and the mutant strain were compared.The results show that the deletion of vhh gene did not affect the growth of the strain,neither the secretion of extracellular protease,the stress response to H2O2 and Cu2+,the uptake and utilization of iron,and the 15 antibiotics resistance and biofilm formation,but it caused the strain to swim and surge significantly.In addition,although vhh was highly expressed in the wild type strain,the strain did not show hemolytic activity.The results indicate that vhh negatively regulates the bacterial motility.The study provides novel information for understanding the function of vhh gene in V.harveyi. |
| |
| Keywords: | Vibrio harveyi Hemolysin gene vhh Gene knockout Biological characteristics Bacterial motility |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
