| 蒙古黄芪的组织培养及辐射诱变研究 |
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| 引用本文： | 赵秀娟,张红霞.蒙古黄芪的组织培养及辐射诱变研究[J].农业科学与技术,2009,10(2):37-40. |
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| 作者姓名： | 赵秀娟 张红霞 |
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| 作者单位： | 内蒙古科技大学生物与化学工程学院,内蒙古包头,014010
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| 基金项目： | 内蒙古科技大学校内基金 |
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| 摘 要： | 1材料与方法
1．1材料供试材料为内蒙古包头市美岱召协力气村采收的蒙古黄芪的种子，蒙古黄芪无菌苗的叶片、子叶和下胚轴。试剂：0．1％升汞溶液、MS培养基、1mol／LNaOH、1mol／LI-ICI、琼脂、蔗糖、2．0mg／ml6-BA、2．0mg／ml NAA、0．1mg／ml NAA、LS培养基。
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| 关 键 词： | 蒙古黄芪 辐射诱变 组织培养 MS培养基 NAA 材料 包头市 内蒙古 |
Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge.var.mongholicus (Bge.) Hsiao |
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| ZHAO Xiu-juan,ZHANG Hong-xia.Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge.var.mongholicus (Bge.) Hsiao[J].Agricultural Science ＆ Technology,2009,10(2):37-40. |
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| Authors: | ZHAO Xiu-juan ZHANG Hong-xia |
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| Institution: | (Biological and Chemical Engineering School, Inner Mongolia University of Science and Technology, Baotou 014010) |
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| Abstract: | Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A.membranaceus Bge. Method] With the different parts of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao aseptic seedling as explants (leaves, cotyledons, hypocotyls) induced callus, and cotyledon and hypocotyls taken by the method of radiation mutation were studied.Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS +6-BA 2.0 mg/L + NAA 2.0 mg/L, LS +6-BA 2.0 mg/L +NAA 0.1 mg/L, MS + 6-BA 2.0 mg/L + NAA 2.0 mg/L; the optimum mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A.membranaceus Bge. |
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| Keywords: | Astragalus membranaceus Bge var mongholicus (Bge ) Hsiao Tissue culture Radiation mutation |
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