Homogenate extraction technology of camptothecine and hydroxycamptothecin from Camptotheca acuminata leaves |
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| Authors: | Wei-guo Shi Yuan-gang Zu Chun-jian Zhao Lei Yang |
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| Institution: | (1) Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, 150040, P.R. China |
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| Abstract: | Camptothecine (CPT) and hydroxycamptothecin (HCPT), two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol
concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C. acuminata leaves were determined as homogenate time at 8 min, ethanol concentration at 55% and the ratio of material to liquid at 1:15
(g:mL). By using the optimized parameters, we obtained 0.639‰ extraction rate for CPT and 0.437‰ for HCPT. The extraction
yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic,
reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting
CPT and HCPT from C. acuminata leaves, with characteristics of less extraction time and high yield.
Foundation project: This research was supported by National Key Technology R & D Program (grant no. 2006BAD18B04).
Biography: SHI Wei-guo (1970–), male, Ph. D. candidate in Northeast Forestry University, Harbin 150040, P. R. China.) |
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| Keywords: | homogenate Camptotheca acuminata leaves camptothecine hydroxycamptothecin |
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