Evaluation of the live vaccine efficacy of virulence plasmid-cured,and phoP- or aroA-deficient Salmonella enterica serovar Typhimurium in mice |
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| Authors: | Hidenori MATSUI Yasunori ISSHIKI Masahiro EGUCHI Yohsuke OGAWA Yoshihiro SHIMOJI |
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| Institution: | 1)Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5–9–1 Shirokane, Minato-ku, Tokyo 108-8641, Japan;2)Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan;3)National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1–5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan |
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| Abstract: | We evaluated the protective efficacy of 94-kb virulence plasmid-cured, and phoP- or aroA-deficient strains of Salmonella enterica serovar Typhimurium (ΔphoP or ΔaroA S. Typhimurium) as oral vaccine candidates in BALB/c mice. Two weeks after the completion of 3 oral immunizations with 1 × 108 colony-forming units (CFU) of virulence plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium at 10-day intervals, S. Typhimurium lipopolysaccharide (LPS)-specific mucosal secretory immunoglobulin A (s-IgA) antibody titers were detected in the cecal homogenate, bile and lung lavage fluid, but not in the intestinal lavage fluid. In addition, the increases in S. Typhimurium LPS-specific immunoglobulin G (IgG) and IgA antibody titers in the serum were also observed 2 weeks after completing 3 oral immunizations with virulence
plasmid-cured, and ΔphoP or ΔaroA S. Typhimurium. The series of 3 oral immunizations protected the mice against an oral challenge with 5 × 108 CFU of the virulent strain of S. Typhimurium, suggesting that both the virulence plasmid-cured, and ΔphoP and ΔaroA S. Typhimurium strains are promising candidates for safe and effective live S. Typhimurium vaccines. |
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| Keywords: | aroA BALB/c mouse oral vaccine phoP Salmonella entericaserovar Typhimurium |
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