| 南洞庭湖15种野生蚌类遗传多样性分析 |
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| 引用本文： | 许宝红,肖调义,苏建明,江辉,葛熹凯.南洞庭湖15种野生蚌类遗传多样性分析[J].湖南农业大学学报(自然科学版),2009,35(1). |
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| 作者姓名： | 许宝红 肖调义 苏建明 江辉 葛熹凯 |
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| 作者单位： | 1. 农业大学动物科学技术学院,湖南,长沙,410128
2. 农业大学物医学院,湖南,长沙,410128 |
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| 基金项目： | 湖南省科学技术厅攻关项目，湖南省教育厅重点项目，湖南农业大学青年基金 |
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| 摘 要： | 采用RAPD技术,选用11条有效随机引物研究了南洞庭湖15种野生蚌(圆顶珠蚌、尖锄蚌、圆头楔蚌、鱼尾楔蚌、巨首楔蚌、射线裂脊蚌、球形无齿蚌、勇士尖嵴蚌、扭蚌、杜氏珠蚌、背瘤丽蚌、短褶矛蚌、三巨瘤丽蚌、环带丽蚌、蚶形无齿蚌)之间的遗传多样性,得到RAPD-DNA扩增条带1 775条,片段长度500～1 500 bp,平均每条引物扩增出43.5条带,这些条带构成了南洞庭湖15种野生蚌的遗传多样性图谱.分析表明,属间的遗传距离以裂脊蚌属和尖嵴蚌属间最近,为0.485 7,丽蚌属与其他属间的遗传距离最远,为0.729 2;种间的遗传距离以圆头楔蚌和巨首楔蚌间最小,为0.333 3,背瘤丽蚌与三巨瘤丽蚌及环带丽蚌的遗传距离最大,为0.678 2.从种属关系归属来看,丽蚌属和尖锄蚌属归属于小方蚌亚科;无齿蚌属归属于无齿蚌亚科;矛蚌属、扭蚌属、裂脊蚌属、尖嵴蚌属、楔蚌属和珠蚌属同归属于珠蚌亚科.
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| 关 键 词： | 蚌 遗传多样性 南洞庭湖 |
Genetic diversity based on RAPD of the fifteen wild freshwater mussel species in South Dongting Lake |
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| XU Bao-hong,XIAO Tiao-yi,SU Jian-ming,JIANG Hui,GE Xi-kai.Genetic diversity based on RAPD of the fifteen wild freshwater mussel species in South Dongting Lake[J].Journal of Hunan Agricultural University,2009,35(1). |
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| Authors: | XU Bao-hong XIAO Tiao-yi SU Jian-ming JIANG Hui GE Xi-kai |
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| Institution: | a.College of Animal Science and Technology;b. College of Veterinary Medicine;HNAU;Changsha 410128;China |
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| Abstract: | RAPD technology was used to study fifteen wild freshwater mussel species in South Dongting Lake, and 11 random primers were selected . 1 775 RAPD bands have been obtained, length between 500 bp and 1 500 bp, its band rate of primer 43.5, these bands have revealed genetic diversity maps of the fifteen wild freshwater mussel species in south dongting lake. Genetic analysis of these mussels indicated, the closest genetic distance among genera is 0.485 7, between Schistodesmus and Acuticosta, the farthest is 0.... |
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| Keywords: | RAPD |
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