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| 贵州地方水稻品种“禾”的SSR指纹图谱构建 | |
| 引用本文： | 马琳,余显权,赵福胜.贵州地方水稻品种“禾”的SSR指纹图谱构建[J].西南农业学报,2010,23(1). |
| 作者姓名： | 马琳 余显权 赵福胜 |
| 作者单位： | 1. 贵州大学,生命科学院,贵州,贵阳,550025 2. 贵州大学,教学实验场,贵州,贵阳,550025 3. 贵州大学,水稻研究所,贵州,贵阳,550025 |
| 基金项目： | 国家科技部科技支撑项目，贵州省科技厅项目"水稻种质资源创新和超级品种选育"，贵州大学研究生创新基金项目"贵州禾遗传多样性分析及典型品种的指纹图谱构建" |
| 摘 要： | 利用SSR标记对24份"禾"品种进行遗传多样性分析,初步建立了其DNA指纹图谱。从145对SSR引物中筛选出21对多态性引物组合,对所有供试材料进行分析,共检测出73个等位基因,平均为3.4762;平均PIC指数为0.4084,范围在0.1175(RM147)~0.6810(RM336)。应用6对引物(RM20A、RM161、RM253、RM280、RM336和RM495)的组合获得了每个品种的SSR特征带,可以将所有供试材料区分开来,从而建立24份"禾"品种的SSR指纹图谱。UPGMA聚类分析表明,在相似系数为0.652处,可将所有材料分为5类,聚类结果表明品种间的亲缘关系与地理来源关系不大。 |
| 关 键 词： | 地方水稻品种 "禾" 指纹图谱 贵州 |
Establishment of SSR Fingerprint Map of Local Rice Varieties 'He' in Guizhou |
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| MA Lin,YU Xian-quan,ZHAO Fu-sheng.Establishment of SSR Fingerprint Map of Local Rice Varieties 'He' in Guizhou[J].Southwest China Journal of Agricultural Sciences,2010,23(1). | |
| Authors: | MA Lin YU Xian-quan ZHAO Fu-sheng |
| Institution: | MA Lin1,YU Xian-quan2,ZHAO Fu-sheng3 (1.College of Life Sciences,Guizhou University,Guizhou Guiyang 550025,China,2.Teaching Experiment Farm,3.Rice Research Institute,China) |
| Abstract: | The DNA fingerprint map of 24 local rice varieties was established from analyzing the genetic diversity by using SSR marker in Guizhou.73 alleles were detected from tested rice varieties by using 21 pairs of primer combinations with polymorphism screened from 145 pairs of SSR primers.The average number of alleles per locus was 3.4762 and its average PIC index with 0.4084 varied from 0.1175(RM147)-0.6810(RM336).The SSR fingerprint maps of 24 local rice varieties were established according to the SSR characte... |
| Keywords: | SSR Local rice variety 'He' SSR Fingerprint Guizhou |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |