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| 反相高效液相色谱检测低浓度5-氨基乙酰丙酸 | |
| 引用本文： | 张龙涛,李昂,史怀,朱育菁,缪伏荣,冯玉兰,刘波.反相高效液相色谱检测低浓度5-氨基乙酰丙酸[J].中国农学通报,2013,29(12):164-167. |
| 作者姓名： | 张龙涛 李昂 史怀 朱育菁 缪伏荣 冯玉兰 刘波 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013 2. 中国石油大学化工学院,山东青岛,266300 3. 福建省农业科学院农业生物资源研究所,福州,350003 |
| 基金项目： | 福建省属公益类科研院所基本科研专项"动物铁代谢调控关键因子生物法制备及应用研究"，福建省自然科学基金"δ-氨基乙酰丙酸对动物铁代谢调控的机理研究"，福建省科技重大专项"动物营养免疫调控技术集成及功能性饲料开发" |
| 摘 要： | 建立了一种柱前衍生反相高效液相色谱检测溶液中低浓度5-氨基乙酰丙酸的方法，填补了低浓度ALA的HPLC法检测的空白。使用Agilent SB-C18型色谱柱(250 mm×4.6 mm i.d.，5 μm)，以50 mM甲醇-醋酸钠缓冲溶液(pH 4.0)洗脱，流速为1 mL/min，柱温30℃，254 nm波长下检测10 min。在此条件下，5-氨基乙酰丙酸在浓度为1~1000 mg/L范围内与色谱峰面积值之间线性关系良好，回归系数均在0.9999以上。发酵液产物的加标回收率平均值为101.7%。将检测结果与分光光度法相比，符合较好。测试数据表明，此方法简单、可靠，对于低浓度的ALA测定效果良好。 |
| 关 键 词： | 籽粒充实 籽粒充实 |
| 收稿时间： | 2012/8/11 0:00:00 |
| 修稿时间： | 2012/10/11 0:00:00 |
Determination of Low Concentration 5-Aminolevulinic Acid by Reversed Phase High Performance Liquid Chromatography |
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| Zhang Longtao , Li Ang , Shi Huai , Zhu Yujing , Miao Furong , Feng Yulan , Liu Bo.Determination of Low Concentration 5-Aminolevulinic Acid by Reversed Phase High Performance Liquid Chromatography[J].Chinese Agricultural Science Bulletin,2013,29(12):164-167. | |
| Authors: | Zhang Longtao Li Ang Shi Huai Zhu Yujing Miao Furong Feng Yulan Liu Bo |
| Abstract: | A reversed phase high performance liquid chromatography with precolumn derivatization was developed for the determination of low concentration 5-aminolevulinic acid (ALA). An Agilent SB-C18 column (250 mm ×4.6 mm i.d., 5μm) was used for separation, and the elution was performed with methanol and 50 mM sodium acetate solution (adjustment of pH to 4.0 with acetic acid). The flow rate was 1 mL.min-1, the temperature of column was 30℃, and the detection wavelength was at 254 nm with the detection time of 10 minutes. The dynamic linear range is 1-1000 mg.L-1, and the correlation coefficient is above 0.9999. The average recoveries of ALA in 3 samples of fermentation were 101.7%. The results agreed well with those of spectrophotometry. So the developed method is simple and reliable for the determination of low concentration ALA. |
| Keywords: | low concentration |
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