| 便携式土壤土霉素提取-检测装置设计与试验 |
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| 引用本文： | 张航,高信,刘倩,由天艳.便携式土壤土霉素提取-检测装置设计与试验[J].农业机械学报,2024,55(4):32-39. |
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| 作者姓名： | 张航 高信 刘倩 由天艳 |
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| 基金项目： | 江苏高校优势学科建设工程(三期)项目(PAPD-2018-87)、中国博士后科学基金项目（2022M713499）和江苏大学高级人才科研启动基金项目（JDG19026） |
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| 摘 要： | 土壤中土霉素（Oxytetracycline，OTC）高性能的现场分析方法对于保护生态环境安全和维护人类健康具有重要意义。针对土壤中痕量OTC的现场检测难题，设计功能集成的便携式提取-检测装置，用于土壤中OTC的现场提取与精准分析。首先，基于集成电路技术与3D建模，研制具有称量、搅拌与离心功能的便携式装置，并通过与实验室用提取装置的性能对比，验证装置的提取精度；分别以LED和便携式电化学工作站为光电化学检测的光源驱动和信号采集装置，研制便携式检测装置；进行土壤中OTC的现场分析试验。结果表明，研制的便携式装置对土壤OTC的提取精度较高，检测的线性范围为1×10-8~1×10-6mol/L，检出限为5.33×10-9mol/L；在现场分析试验中，对土壤中OTC检测的加标回收率为92%~97%，相对标准偏差为1.8%~5.2%，且准确度得到了国标法的验证。
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| 关 键 词： | 土壤土霉素 便携式装置 提取 现场检测 光电化学传感器 |
| 收稿时间： | 2023/12/6 0:00:00 |
Design and Experiment on Portable Device for Extraction and Detection of Oxytetrarycline in Soil |
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| ZHANG Hang,GAO Xin,LIU Qian,YOU Tianyan.Design and Experiment on Portable Device for Extraction and Detection of Oxytetrarycline in Soil[J].Transactions of the Chinese Society of Agricultural Machinery,2024,55(4):32-39. |
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| Authors: | ZHANG Hang GAO Xin LIU Qian YOU Tianyan |
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| Institution: | Jiangsu University |
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| Abstract: | Developing portable devices for extraction and detection of oxytetracycline (OTC) in soil, and developing accurate methods for on-site analysis of OTC in soil is of great importance to ensure the safety of agricultural environment and human health. The existing devices for OTC extraction require additional devices to meet the needs for on-site extraction, and the low sensitivity of the on-site detection method limits its application in accurate detection of trace OTC in soil. Integration circuits provide ideas of hardware integration design for soil OTC extraction-detection devices because of the advantages of simple circuitry, low failure rate, low power consumption, and high reliability;the photoelectrochemical (PEC) aptasensor is expected to achieve accurate detection of OTC for its high sensitivity, good selectivity, and easy portability. A portable device was developed for extraction and PEC detection of OTC in soil by designing an integrated hardware circuit. Firstly, a portable device for extraction of OTC in soil was designed and developed with STM32F103ZET6 as the control core. The extraction of OTC from soil was accomplished using the developed portable device and laboratory device, respectively, and the extraction precision was verified by using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Subsequently, a portable PEC detection device was prepared by using integrated Bi2S3-C3N4 based screen-printed electrodes, low-power LEDs, and a miniaturized electrochemical workstation. The design of hardware circuit and light source darkroom had the function of controlling the timing switching of the LEDs during PEC detection and avoiding the interference of the external light source on the output PEC signal. Lastly, the portable extraction-PEC detection device was used for on-site analysis of OTC in soil. Results showed that the extraction precision of the developed portable device was accordance with that of the laboratory device;and the portable PEC detection device had a linear range of 1×10-8~1×10-6mol/L and a detection limit of 5.33×10-9mol/L was obtained for OTC detection;and the results of the on-site analytical method for OTC were basically agree with the results of the UPLC-MS/MS method. This work would realize the on-site analysis of OTC in soil and provide technological support for soil treatment. |
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| Keywords: | oxytetracycline in soil portable device extraction on-site detection photoelectrochemical sensor |
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