| 山桐子离体培养植株再生 |
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| 引用本文： | 杨恩让,刘晓敏,解 庆.山桐子离体培养植株再生[J].西北林学院学报,2013,28(6):95-98. |
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| 作者姓名： | 杨恩让 刘晓敏 解 庆 |
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| 作者单位： | 1.宝鸡市林业科学研究所,陕西 宝鸡 721000; 2.西北农林科技大学,林学院 陕西 杨陵 712100 |
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| 摘 要： | 以种子和叶片为外植体,研究了离体条件下外植体类型及植物生长调节剂对山桐子器官发生和植株再生的影响。结果表明:种子为诱导愈伤组织的适宜外植体; 在MS+1.0 mg·L-16-BA +1.0 mg·L-1 NAA +3%蔗糖+0.6%琼脂的培养基上,愈伤诱导率达96.8%; 继代培养在MS+0.08 mg·L-1 TDZ + 1.5 mg·L-16-BA +0.05 mg·L-1 NAA 培养基上能成功的诱导芽的分化,诱导率达48.3%以上; 芽增殖的适宜培养基为1/2MS +0.03 mg·L-1 TDZ +2.0 mg·L-16-BA +0.1 mg·L-1 NAA +3%蔗糖+0.6%琼脂,增殖系数为4.83; 在1/2MS+ 0.5 mg·L-1 IBA +2%蔗糖+0.6%琼脂培养基上,无根苗生根率达88%。
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| 关 键 词： | 山桐子 组织培养 再生体系 |
Establishment of Plantlet Regeneration System of Idesia polycarpa in vitro |
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| YANG En-rang,LIU Xiao-min,XIE Qing.Establishment of Plantlet Regeneration System of Idesia polycarpa in vitro[J].Journal of Northwest Forestry University,2013,28(6):95-98. |
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| Authors: | YANG En-rang LIU Xiao-min XIE Qing |
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| Institution: | 1 Baoji Forestry Science Research Institute, Baoji, Shaanxi 721000, China; 2. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: | Seeds and tender leaves of Idesia polycarpa were used as a source of initial explants. The effects of explants and plant growth regulators on organogenesis and plantlet regeneratio in vitro were studied. The results showed that seeds were more suitable for initial explants in inducing calli. High induction rate of 96.8% was achieved in MS medium supplemented with 1.0 mg·L-1 NAA,1.0 mg·L-1 6-BA, 3% sucrose and 0.6%agar. MS medium supplemented with 0.08 mg·L-1 TDZ, 1.5 mg·L-1 6-BA and 0.05 mg·L-1 NAA in subculture was in favor of the shoot differentiation and the rate was over 48.3%. The shoot proliferation coefficient reached 4.83 in 1/2MS medium with 0.03 mg·L-1TDZ, 2.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA,3% sucrose and 6% agar. High rooting percentages and excellent development root systems were obtained on 1/2MS medium with 0.5 mg·L-1 IBA, 2% sucrose and 0.6% agar. |
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| Keywords: | Idesia polycarpaIdesia polycarpa tissue culture tissue culture regeneration system |
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