| 施氏鲟性别相关的微卫星标记筛选 |
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| 引用本文： | 刘洋,李超,刘翠,鲁翠云,赵元凤,孙效文.施氏鲟性别相关的微卫星标记筛选[J].鲑鳟渔业,2014(2):9-13,19. |
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| 作者姓名： | 刘洋 李超 刘翠 鲁翠云 赵元凤 孙效文 |
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| 作者单位： | [1]大连海洋大学水产与生命学院,辽宁大连116023 [2]中国水产科学研究院黑龙江水产研究所,淡水鱼类育种国家地方联合工程实验室,黑龙江哈尔滨150070 |
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| 基金项目： | 公益性行业（农业）科研专项（200903046-07）;国家科技基础条件--水产种质资源平台. |
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| 摘 要： | 用32个微卫星标记分析了30尾（15尾♀；15尾♂）性成熟（12龄）施氏鲟（Acipenser schrenckii）雌雄个体的遗传差异。结果表明：雌、雄群体的遗传多样性差异没有达到显著水平。在6个微卫星标记中有9个等位基因在雌、雄群体中分布比例差异达到50%以上，其中雌性群体HLJSX 194-239bp、HLJSX201-207bp、HLJSX209-210bp、HLJSX210-178bp和HLJSX215-221bp 5个等位基因频率显著高于雄性群体；雄性群体HLJSX194-184bp、HLJSX201-214bp、HLJSX210-233bp和HLJSX226-194bp 4个等位基因频率显著高于雌性群体，初步表明这些基因位点可能与性别决定位点存在一定连锁关系。本研究结果为施氏鲟早期性别鉴定积累了基础数据。
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| 关 键 词： | 施氏鲟 性别相关标记 微卫星 |
Screening of Sex-related Microsatellite Markers in Amur Sturgeon（Acipenser schrenckii） |
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| Authors: | LIU Yang LI Chao LIU Cui LU Cui-yun ZHAO Yuan-feng SUN Xiao-wen |
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| Institution: | 1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongiiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China) |
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| Abstract: | In this paper, 33 microsatellite markers were used to screen the sex-related DNA markers through analyzing DNA differ-ences between female and male in 12 years old mature Amur sturgeon （Acipenser schrenckii） （ 15 samples）, The results showed that there were no significant differences between the female and the male populations. However, 9 alleles derived from 6 SSR loci showed different distributions between female and male groups at a rate of 〉50%. Five alleles HLJSX194-239bp, HLJSX201-207bp, HLJSX 209-210bp, HLJSX210 178bp, and HLJSX215-221 bp were found to be significantly higher frequency in the female samples than those in the male samples. However, there was significantly higher frequency in 4 alleles HLJSX194-184bp, HLJSX201-214bp, HLJSX210-233bp, and HLJSX226-194bp in the male samples than those in the female samples, indicating that these loci are linked with sex-determinate loci. The findings provide a basis for gender identification during early development of Amur sturgeon. |
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| Keywords: | Amur sturgeon sex-related markers microsatellite |
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