| 苹果茎痘病毒RT-PCR检测及分子变异分析 |
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| 引用本文： | 李丽丽,董雅凤,张尊平,张志宏,范旭东,裴光前.苹果茎痘病毒RT-PCR检测及分子变异分析[J].园艺学报,2010,37(1):9-16. |
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| 作者姓名： | 李丽丽 董雅凤 张尊平 张志宏 范旭东 裴光前 |
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| 作者单位： | ( 沈阳农业大学园艺学院, 沈阳110161; 中国农业科学院果树研究所, 辽宁兴城125100) |
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| 基金项目： | 中国农业科学院科研基金项目(农科院[2006]31号) |
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| 摘 要： | 利用RT-PCR技术对采自6个苹果品种和7个梨品种的33个样本中的苹果茎痘病毒(Applestem pitting virus,ASPV)进行了检测,ASPV感染率为63%(19/33)。对6个ASPV中国分离物外壳蛋白(Coat protein,CP)基因进行了扩增、克隆和测序,测序结果显示CP基因全长为1191nt,编码397个氨基酸。中国分离物与GenBank中其他分离物核苷酸序列同源性为70.2%~91.7%。系统进化分析显示不同ASPV分离物分为3个大的类群,呈现一定的寄主相关性,并未呈现明显的地理相关性。第一和第三大类群的寄主皆为苹果,第二大类群包含6个梨分离物和2个苹果分离物。不同ASPV分离物的抗原指数差异较大,推断可能由于序列变异引起抗原表位变异,从而引起血清型差异。
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| 关 键 词： | 苹果 梨 苹果茎痘病毒 RT-PCR检测 序列分析 |
| 收稿时间： | 2009-9-7 |
| 修稿时间： | 2009-11-11 |
RT-PCR Detection and Molecular Variability of Apple stem pitting virus |
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| LI Li-li,DONG Ya-feng,ZHANG Zun-ping,ZHANG Zhi-hong,FAN Xu-dong,PEI Guang-qian.RT-PCR Detection and Molecular Variability of Apple stem pitting virus[J].Acta Horticulturae Sinica,2010,37(1):9-16. |
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| Authors: | LI Li-li DONG Ya-feng ZHANG Zun-ping ZHANG Zhi-hong FAN Xu-dong PEI Guang-qian |
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| Institution: | (College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China ) |
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| Abstract: | Thirty-three samples including 6 apple and 7 pear cultivars were detected for Apple stem pitting virus(ASPV)by RT-PCR,the infected rate was up to 63%(19/33).The ASPV coat protein(CP)gene of 6 Chinese isolates were amplified by RT-PCR,cloned and sequenced.Sequence analysis showed that the full-length CP gene comprised 1 191 nt and encoded 397 amino acid.Identities of nucleotide between Chinese isolates and other isolates were 70.2%-91.7%.Phylogenetic analysis showed that all isolates of ASPV fell into three ... |
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| Keywords: | apple pear Apple stem pitting virus RT-PCR detection sequence analysis |
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