| 甘蓝苗期多抗性鉴定技术研究 |
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| 引用本文： | 王超,吴世昌.甘蓝苗期多抗性鉴定技术研究[J].东北农业大学学报,2000,31(2):152-159. |
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| 作者姓名： | 王超 吴世昌 |
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| 作者单位： | 东北农业大学农学院园艺系,东北农业大学农学院园艺系,东北农业大学农学院园艺系,东北农业大学农学院园艺系 黑龙江 哈尔滨150030,黑龙江 哈尔滨150030,黑龙江 哈尔滨150030,黑龙江 哈尔滨150030 |
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| 摘 要： | 甘蓝苗期多抗性鉴定是甘蓝多抗性育种的基础,对抗源的筛选有实际应用价值。本项试验,选用４份甘蓝育种自交系材料,１０３,３０２,８４－１０３５－７和９０－１０７２－７。ＴｕＭＶ 选用“甘－８３－８号”毒株,ＣＭＶ选用“甘９１－２３号”毒株,黑腐病菌（Ｘａｎｔｈｏｍｏｎａｓ ｃａｍｐｅｓｔｒｉｓｐｖ．ｃａｍｐｅｓｔｒｉｓ）选用Ｘ_（０－１）菌株。ＴｕＭＶ与ＣＭＶ分别采用人工摩擦的接种方法,黑腐病菌采用喷雾的接种方法,浓度为１０~８个细菌／ｍＬ。试验设有６个接种处理方法①子叶期接种ＴｕＭＶ,第一片真叶接种ＣＭＶ;②子叶期接种ＣＭＶ,第一片真叶接种ＴｕＭＶ;③子叶期一片接种ＴｕＭＶ,另一片接种ＣＭＶ;④子叶期接种ＴｕＭＶ＋ＣＭＶ的混合液;⑤子叶期单接ＣＭＶ;③子叶期单接ＴｕＭＶ;以上６种处理方法分别在５～６片真叶期喷雾接种黑腐病菌。试验３次重复,在防虫温室内进行３个月的时间。接种后温度维持在１０～３０℃,阴天补充光照,待发病后进行抗病性鉴定。试验结果表明第一种接种处理方法,４份试材的发病程度较其它五种都重,另外试材１０３－１,Ａ_（２０）,８４－１０３８－１１８６,６０６－１２－２１－１４,８４－１０７２－３－４等自交系较
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| 关 键 词： | 甘蓝 苗期 多抗性 鉴定技术 |
Study on the methods of screening for multiple disease resistance in cabbage seedling |
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| WANG Chao,WU Shi-cang,Qin Zhi-wei,LIU Jian-dong.Study on the methods of screening for multiple disease resistance in cabbage seedling[J].Journal of Northeast Agricultural University,2000,31(2):152-159. |
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| Authors: | WANG Chao WU Shi-cang Qin Zhi-wei LIU Jian-dong |
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| Abstract: | The screening for multiple disease resistant resources in cabbage play important role in breeding for multiple disease resistant cabbage and is of great value to practical utilization. 4 inbred lines of cabbage were used as the experimental muterials. They are 103, 302, 84-1035- 7 and 90-1072-7. "Gan-83-8" strain of TuMV, "Gan-91-23" strain of CMV, and X_(0-1) strain of Black rot were employed as the inoculums. The seedlings of cabbage are inoculated with TuMV and CMV, by means of the method of rubbing, Black rot by means of the method of spraying. There were 6 treatments in the experiment. the results of the experiment showed that. 1_(st) treatment was the best practical in the six treatments. The inbred lines such as 103-1, 84-1038-1186, 606-12-21-14 and 84-1072-3-4 el are the multiple disease resistant materials. |
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| Keywords: | screen multiple disease resistance inoculation cabbage |
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