| AFLP在濒危植物岷江柏中的应用初探 |
| |
| 引用本文： | 姚莉,王丽,唐铭霞.AFLP在濒危植物岷江柏中的应用初探[J].西南农业大学学报,2005,27(4):547-550. |
| |
| 作者姓名： | 姚莉 王丽 唐铭霞 |
| |
| 作者单位： | 四川大学,生命科学学院,四川成都610064 |
| |
| 基金项目： | 中国科学院重点项目基金资助（KSCX 2-104） |
| |
| 摘 要： | 以14个岷江柏野生居群个体为试材,对AFLP分析过程中的影响因子（酶切与连接、预扩增、选择性扩增和银染）进行了研究.筛选出4对扩增稳定且多态性丰富的AFLP引物;得到扩增条带274条,多态性带137条（占50.00%）.
|
| 关 键 词： | 岷江柏 AFLP 影响因素 |
| 文章编号： | 1000-2642（2005）04-0547-04 |
| 收稿时间： | 2004-10-20 |
| 修稿时间： | 2004-10-20 |
APPLICATION OF AFPL MARKERS IN GENETIC DIVERSITY STUDY OF THE ENDANGERED PLANT SPECIES CUPRESSUS CHENGIANA |
| |
| YAO Li, WANG Li, TANG Ming- xia.APPLICATION OF AFPL MARKERS IN GENETIC DIVERSITY STUDY OF THE ENDANGERED PLANT SPECIES CUPRESSUS CHENGIANA[J].Journal of Southwest Agricultural University,2005,27(4):547-550. |
| |
| Authors: | YAO Li WANG Li TANG Ming- xia |
| |
| Institution: | College of Life Science, Sichuan University, Chengdu, Sichuan,610064, China |
| |
| Abstract: | AFLP fingerprinting was conducted to study 14 individuals of Cupressus chengiana S.Y. Hu. Essential factors in DNA extraction, restriction, adapter ligation, pre -amplification, selective amplification and silver staining were analyzed. Four primer combinations with stable amplification and rich polymorphism for AFLP were screened, which had 274 distinct and strong amplification bands with a bright background and 137 of them were polymorphic bands, accounting about 50.00% of the total. |
| |
| Keywords: | Cupressus chengiana S Y Hu AFLP influential factor |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《西南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南农业大学学报》下载免费的PDF全文 |
|
