| 呼肠孤病毒B3分离株感染番鸭的病理组织学研究 |
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| 引用本文： | 吴宝成,姚金水,陈家祥,卢惠明,陈枝华.呼肠孤病毒B3分离株感染番鸭的病理组织学研究[J].福建农林大学学报(自然科学版),2001,30(4). |
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| 作者姓名： | 吴宝成 姚金水 陈家祥 卢惠明 陈枝华 |
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| 作者单位： | 1. 福建农林大学动物科学学院,
2. 福建省出入境检疫局, |
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| 摘 要： | 番鸭呼肠孤病毒 B3分离株可经滴鼻、饮水、肌肉和爪垫注射与同居感染 1日龄敏感番鸭 ,潜伏期 3- 11d,死亡率 10 0 % ;接种 1日龄番鸭、半番鸭和雏鸡爪垫可引起注射部位炎性反应 ,爪垫和肌肉注射 1日龄半番鸭和雏鸡不死亡 .病毒感染番鸭主要引起肝、脾、心肌、肾、腔上囊、腺胃、肠粘膜下层等组织局灶性坏死 ;肝、脑血管周围和肾间质、肺间质、心肌间有淋巴样细胞或 /和吞噬细胞聚集 ,法氏囊淋巴细胞变性和坏死 .电镜观察表明 ,感染胚肝细胞和脾淋巴细胞胞浆内有大量病毒样颗粒及近核包涵体 ,感染细胞多核化、空泡化及颗粒化 ;发现含有病毒样颗粒的凋亡细胞 ;吞噬细胞胞浆内有病毒样颗粒和含病毒样颗粒的凋亡小体 .此项研究为国内首次报道
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| 关 键 词： | 番鸭 呼肠孤病毒 肝“白点病” 病理学 |
Pathology of infection with reovirus isolate B3 in Muscovy ducks |
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| WU Bao cheng ,YAO Jin shui ,CHEN Jia xiang ,LU Hui ming ,CHEN Zhi hua.Pathology of infection with reovirus isolate B3 in Muscovy ducks[J].Journal of Fujian Agricultural and Forestry University,2001,30(4). |
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| Authors: | WU Bao cheng YAO Jin shui CHEN Jia xiang LU Hui ming CHEN Zhi hua |
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| Institution: | WU Bao cheng 1,YAO Jin shui 1,CHEN Jia xiang 1,LU Hui ming 1,CHEN Zhi hua 2 |
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| Abstract: | The one day old Muscovy ducks could be infected with the reovirus isolate B 3 by nasal, oral, intramuscular (i.m.), pad injection and co breeding. The ncubation period of one day old Muscovy ducks inoculated with the isolate B 3 was from 3 to 11 days, the rate of mortality was up to 100%, but some resistance to the isolate B 3 were found survive among the one day old hybrid (Muscovy) ducks or chickens. There were inflammatory responses caused by the pad inoculation to the Muscovy ducks, hybrid (Muscovy) ducks or chickens of one day old, though the apparent clinical signs didn't appear by i.m. injection on hybrid (Muscovy) ducks or chickens. Macroscopically, fibrinous pericarditis, gray white necrotic foci, enlarged and friable liver, and marbled spleen were seen in dead Muscovy ducks with the isolate B 3. By microscopical observation, it was found that there were focal necroses in liver, spleen, cardiac muscle (heart), kidney, bursa, proventriculus and intestinal submucosal coat. Lymphocytic and phagecytic infiltration were consistently found in the perivascular of the liver and brain, and in the interstice of cardiac muscle, kidney and lung. The lymphocytic denaturations and necroses were also seen in the bursa of Fabricius. EM examinations revealed that there were virus like particles and inclusion bodies near the nucleus; multinuclear, vacuolization and granular degeneration were found in the liver cells and the lymphocytes of spleen of infected embryos. The isolate B 3 also resulted in apoptosis, virus like particles and apoptic bodies in the cytoplasm of phagocytes. The findings were reported for the first time in China. |
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| Keywords: | Muscovy duck reovirus liver white spots disease pathology |
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