| 三七粉中总黄酮的检测 |
| |
| 引用本文： | 李忠英,罗跃中.三七粉中总黄酮的检测[J].安徽农业科学,2010,38(27):14942-14943. |
| |
| 作者姓名： | 李忠英 罗跃中 |
| |
| 作者单位： | 湖南化工职业技术学院,湖南株洲,412004;湖南化工职业技术学院,湖南株洲,412004 |
| |
| 摘 要： | 目的]为三七总黄酮的开发利用提供科学依据。方法]以三七粉为原料,采用50%乙醇溶解、超声波提取法提取其中的总黄酮,以芦丁为标准品,采用紫外分光光度法测定提取液中总黄酮的含量。结果]芦丁的最大吸收波长为510nm;在0.010～0.997mg/ml范围内,样品浓度与其吸光度具有良好的线性关系;方法的加样回收率为99.58%,RSD为1.22%;重复性试验RSD为2.92%;精密度试验RSD为3.23%。说明该方法测定结果准确,重复性好。结论]建立了以芦丁为标准品的三七粉中总黄酮含量的检测方法。
|
| 关 键 词： | 三七粉 超声波 总黄酮 检测 |
Detection of Total Flavonoids in Panax notoginseng Powder |
| |
| Institution: | LI Zhong-ying et al(Hunan Chemical Vocational Technology Institute,Zhuzhou,Hunan 412004) |
| |
| Abstract: | Objective] The study was to provide the scientific basis for the development and utilization of total flavones in Panax notoginseng.Method] P.notoginseng powder was taken as the raw material.The total flavonoids in it was extracted by 50% ethanol dissolution and ultrasonic extraction method.The content of total flavonoids in the extract was measured by UV spectrophotometry with rutin as the standard sample.Results] The maximum absorption wavelength of rutin was 510 nm.The linear relation between sample concns.and absorbance was good in the range of 0.010-0.997 mg/ml.The average recovery of the method was 99.58% with RSD of 1.22%.The RSD of the repeatability experiment was 2.92% and that of accuracy experiment was 3.23%.Which indicated that the method was accurate and repeatable.Conclusion]The study established a detection method of total flavonoids in P.notoginseng powder with rutin as the standard sample. |
| |
| Keywords: | Panax notoginseng powder Ultrasonic Total flavonoids Detection |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
