Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway |
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| Authors: | DING Gui-xia ZHANG Ai-hua HUANG Song-ming WU Yuan-jun FEI Li GUO Mei CHEN Rong-hua |
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| Institution: | 1. Department of Pediatrics, Second Hospital of Nanjing Medical University, Nanjing 210011, China;
2. Center of Pedicatric Nephrology, Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE2 concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE2 production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway. |
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| Keywords: | Kidney Mesangial cell NF-κB Prostaglandin-endoperoxide synthase |
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