Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro |
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| Authors: | XIE Tong WU Ping TANG De-shen JIANG Li-ming |
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| Institution: | 1. Institute of Nephrology, Guangdong Medical College, Zhanjiang, 524001, China;
2. the Central Laboratory, Guangdong Medical College, Zhanjiang, 524001, China |
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| Abstract: | AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level. |
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| Keywords: | Cyclophosphamide Apoptosis Glomerular mesangial cells Rats |
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