Relationship of p21WAF1 gene polymorphisms with protein expression in breast carcinomas |
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| Authors: | XIAO Ning-xin LI Zhong-hong JIN Yi WANG Guo-qiang SU Zu-lan |
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| Institution: | 1. Department of Pathology, The People's Hospital of Jiangmen, Guangdong 529050, China;
2. Department of General Surgery, The People's Hospital of Jiangmen, Guangdong 529050, China;
3. Department of Pathology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China;
4. Department of Information, The People's Hospital of Jiangmen, Guangdong 529050, China |
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| Abstract: | AIM: To investigate the relationship between p21WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ2=3.94, P＜0.05). The positive immunohistochemical reaction of p21WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ2=16.84, P＜0.01). The positive immunohistochemical reaction of p21WAF1 protein were found in 100% (18/18) of breast carcinomas with p21WAF1 gene polymorphisms and 39% (32/82) of no p21WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ2=21.95, P＜0.01). The p21WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules. |
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| Keywords: | Breast neoplasms Genes p21 Protein p21 Polymorphism |
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