Effects of NF-κB "decoy" oligodeoxynucleotides on TNF-α and IL-6 expression in LPS-induced mouse macrophages |
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| Authors: | WANG Jian DENG Xiao-ming REN Xu-yi LI Jin-bao CAO Jin-mei |
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| Institution: | 1. Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Deaprtment of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | AIM: To study the effect of NF-κB "decoy" oligodeoxynucleotides on TNF-α and IL-6 expression in LPS-induced mouse macrophages. METHODS: Mouse macrophage cell line J774.1 cells were cultured with LPS and liposome-mediated oligodeoxynucleotides, and the levels of TNF-α and IL-6 measured in the different culture supernatant by enzyme linked immunosorbent assay. RNA was extracted from macrophages, and the mRNA expression of TNF-α and IL-6 in macrophages was observed by RT-PCR. RESULTS: NF-κB "decoy" oligodeoxynucleotides decreased the expression of TNF-α and IL-6 in LPS-induced macrophages and inhibited generation of TNF-α and IL-6. The level of TNF-α and IL-6 did not change in control group. CONCLUSIONS: NF-κB "decoy" oligodeoxynucleotides inhibit the expression of TNF-α and IL-6 in LPS-induced macrophages, which is probably due to the specific inhibition of activated NF-κB binding sites . |
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| Keywords: | NF-kappa B Oligonucleotides Tumor necrosis factor Interleukin-6 Macrophages |
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