The mechanism of neuronal injury and repair after focal cerebral ischemia |
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| Authors: | ZHANG Zai-qiang LONG Jie LI Xiao-ling |
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| Institution: | Department of Neurology, Beijing Tiantan Hospital Affiliated to Capital University of Medical Science, Beijing 100050, China |
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| Abstract: | AIM:To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic/apyrimidinic endonuclease (APE/Ref-1) after focal cerebral ischemia. METHODS:A model of middle cerebral artery occlusion in rats was performed. The expression of caspase-3P20 and APE/Ref-1 was examined by immunohistochemistry staining, TUNEL was applied to detected DNA damage, and double labeling with TUNEL and APE/Ref-1 was used to determine the relationship between APE/Ref-1 and DNA damage. RESULTS:The active subunit P20 of caspase-3 was predominantly expressed within ischemic penumbra. The peak time of caspase-3P20 positive cells preceded the appearance of TUNEL. With aggravation of cerebral ischemia, APE/Ref-1 immunoreactive cells in penumbra were significantly decreased. CONCLUSION:The activation of caspase enzymatic cascade following cerebral ischemia leads to degradation in DNA, meanwhile, decrease in DNA repair molecules or the failure of DNA repair may deteriorate the course. |
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| Keywords: | Brain ischemia Caspases Neurons DNA Rats |
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