Alteration of p38 MAPK activity in lung tissue in diabetic rats |
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| Authors: | ZHANG Jing-ping LIU Guo-liang |
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| Institution: | 1. Department of Infectious Diseases, The First Hospital, China Medical University, Shenyang 110001, China;
2. Department of Endocrinous Diseases, The First Hospital, China Medical University, Shenyang 110001, China |
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| Abstract: | AIM: To observe the pathologic changes in lung and the role of p38 MAPKinase signal pathways in pulmonary alteration in diabetic rats. METHODS: Diabetic rats were induced by intraperitoneally injected streptozotozin (STZ). After 4 weeks, we observed the pathologic changes in lungs, tested protein kinase C (PKC) activities by isotope in lungs of model rats, tested transforming growth factor (TGF-β1) by Western blotting and immunohistochemical analysis, and determined the expression of p38 MAPKinase mRNA using in situ hybridization.RESULTS: After STZ administration for 4 weeks, we observed thickened pulmonary capillary basal lamina and increased number of fibre in Diabetes mellitus (DM) rats. TGF-β1 levels, PKC and p38 MAPK activities were also found increased. CONCLUSION: The increased activities of TGF-β1 and p38 MAPK suggeste that TGF-β1 may play an important role in diabetic lung, and hyperglycemia-PKC-p38 MAPK signal pathways may be involved in the pathogenesis of diabetes. |
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| Keywords: | Diabetes mellitus Lung Rats Transforming growth factor beta Protein kinase C Mitogen-activated protein kinases |
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