Effects of Bene Jones protein and TGF-β1 on the proliferation of rat renal proximal tubular cells in vitro |
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| Authors: | ZHOU Zhen-hai LI You-ji CHEN Yun-xian LI Xiao-ying YU Xue-qing LI Juan LUO Shao-kai YUAN Yao-guang |
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| Institution: | 1. Department of Medical Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;
2. Department of Medical Hematology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China |
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| Abstract: | AIM:To study the effect of Bene Jones protein (BJP) from multiple myeloma(MM) patient and TGF-β1 on cultured renal proximal tubular cell(PTC) proliferation.METHODS:H3]TdR incorporation was used to study the effect of λBJP and TGF-β1 on cultured rat NRK.52E PTC proliferation, the expression of TGF-β1 in the supernatant of PTC cultured with BJP was assessed with ELISA.RESULTS:① H3]TdR incorporation of PTC was inhibited by BJP in a dose-dependent manner, when co-cultured with 100-800 μmol/L BJP and 2.0 μg/L TGF-β1, the H3]TdR incorporation was lower than that of BJP alone, especially when BJP≥400 μmol/L;②The expression of TGF-β1 in the supernatant of PTC cultured with BJP was increased, especially when BJP≥400 μmol/L(P＜0.05);③ The H3]TdR incorporation of PTC was also inhibited by exogenous TGF-β1 in a dose-dependent manner.CONCLUSION:λBJP has antiproliferative effect on rat PTC in vitro, The effect is related with stimulating the PTC to produce excessive TGF-β1, which also has antiproliferative effect on PTC in some degree. |
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| Keywords: | Bence Jones protein Transforming growth factor beta Renal proximal tubular cell |
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