Influence of caveolin-1 on the ET-1-induced VSMC proliferation |
| |
| Authors: | TAN Zhi CHEN Jian-wen PAN Jing-yun WANG Ting-huai |
| |
| Institution: | 1. Department of Physiology, Zhongshan Medical College of Sun Yat-sen University, Guangzhou 510089, Chin;
2. Department of Experimental Physiological Science, Zhongshan Medical College of Sun Yat-sen University, Guangzhou 510089, China |
| |
| Abstract: | AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The 3H]-thymidine (3H]TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS:The ETA receptor specific antagonist BQ123 inhibited the increase in3H]TdR incorporation in response to ET-1 on VSMC.Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC.After 24 h treatment of VSMC with ET-1,the expression of caveolin-1 in VSMC was significantly decreased.Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC,BQ123 reversed the effect of ET-1.CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor. |
| |
| Keywords: | Caveolins Caveolae Endothelin-1 Muscle smooth vascular |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
