Ginsenoside Rh2 enhances the apoptotic effect ofcisplatin on PC-3M prostatic cancer cells |
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| Authors: | MENG Yan ZHOU Gui-hua LI Yang ZHAO Xue-jian CHEN Yan-ping MA Xing-yuan |
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| Institution: | 1. Department of pathophysiology of Basic Medicine College, Jilin University, Changchun 130021, China;
2. Pharmacology College, Jilin University, Changchun 130021, China |
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| Abstract: | AIM:To study the inducing apoptotic effect of Cisplatin (DDP) combined with Ginsenoside Rh2 on PC-3M prostatic cancer cells in vitro.METHODS:Cell viability was examined by MTT test and the apoptosis of PC-3M by electrophoresis of NP-40 extracting fragmental DNA and flowcytometry.RESULTS:Pc-3M cells were exposed to 15.0 mg/L Rh2 combined with 0.4 mg/L DDP: ①Cell viabililty inhibitory rate was increased by 1.44 times from 25.0% (with 0.4 mg/L DDP) to 61.03%. ②Characteristic apoptotic DNA laddering was found more obviously than using either agent alone. ③Apoptotic rate of PC-3M was increased from 0.32% (with 0.4 mg/L DDP) to 52.2%, which showed similar effect with 4.0 mg/L DDP, it was much higher than that of 15.0 mg/L Rh2 alone (13.6%) (P＜0.01).CONCLUSION:Rh2 greatly enhances the apoptotic effect of DDP on PC-3M prostatic cancer cells. |
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| Keywords: | Apoptosis Ginsenoside Rh2 Cisplatin Prostatic neoplasms |
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