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Inhibitory effect of antisense eukaryotic expression vectors for c-myc on rat airway smooth muscle cells |
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| Authors: | LIU Ying-ge QI Hao-wen LI Huan-zhang SU Ming-quan YU Wen-bin MA Yue-yun |
| Institution: | 1. Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China; 2. Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China |
| Abstract: | AIM: To observe the inhibitory effect of antisense eukaryotic expression vectors for c-myc on rat airway smooth muscle cells. METHODS: Antisense and sense eukaryotic expression vectors for c-myc pcDNA3-myc-antisense and pcDNA3-myc-sense were constructed. Lipofectin was used to introduce antisense and sense eukaryotic expression vectors for c-myc into rat. The inhibitory effect was assayed by MTT cell proliferation assay. Cell cycles were detected by flow cytometry technology. The expression of c-Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense eukaryotic expression vector for c-myc inhibited rat airway smooth muscle cells proliferation. Rat airway smooth muscle cells were prohibited in S phase and the expression of c-Myc was decreased after antisense eukaryotic expression vectors for c-myc were transfected into cells. CONCLUSION: Antisense eukaryotic expression vectors for c-myc inhibit rat airway smooth muscle cell proliferation. |
| Keywords: | Genes myc Muscle smooth Airway Rats |
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