Expression of MIP-1α and RANTES gene and protein in the bronchus of murine asthma |
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| Authors: | ZHANG Wei-xi LI Chang-chong CHENG Xiao-ming CHEN Xiao-fang CAI Xiao-hong DONG Lin WU Rong-xi ZHANG Zheng-xia |
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| Institution: | 1. Division of Pulmonology, Affiliated Yuying Children's Hospital, Wenzhou Medical College, Wenzhou 325027, China;
2. Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China |
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| Abstract: | AIM: To study the role of the gene and protein expression of MIP-1α and RANTES in the bronchus of murine asthma. METHODS: 20 male BALB/C mice were randomly divided into two groups: the control group (A0 group) and asthma group (B0 group). In the experiment, the mice model of asthma was established by the ovalbumin (OVA) challenge methods. The protein expression of MIP-1α and RANTES were detected by immunohistochemistry methods. The gene expressions of MIP-1α and RANTES were detected by in situ hybridization methods. RESULTS: Immunohistochemistry showed that the expressions of MIP-1α protein and RANTES protein around the bronchus of group B0 were significantly higher than those of group A0 (P<0.01), the epithelial cells were the chief expression cells; (2) In situ hybridization showed that the expressions of MIP-1α gene and RANTES gene around the bronchus of group B0 were significantly higher compared to those of group A0 (P<0.01), the epithelial cells were the chief expression cells. CONCLUSION: MIP-1α and RANTES are high expression in the bronchus epithelial cells in experimental murine asthma. |
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| Keywords: | Epithelial cells Asthma Chemokines Mice |
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