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我国部分兰属植物菌根真菌rDNA ITS序列分析
引用本文:李潞滨,胡陶,唐征,庄彩云,刘振静,杨凯,彭镇华.我国部分兰属植物菌根真菌rDNA ITS序列分析[J].林业科学,2008,44(2):160-164.
作者姓名:李潞滨  胡陶  唐征  庄彩云  刘振静  杨凯  彭镇华
作者单位:1. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100,091
2. 北京农学院农业应用新技术北京市重点实验室,北京,102206
基金项目:引进国际先进农业科技计划(948计划),引进国际先进农业科技计划(948计划),北京市属市管高校人才强教计划
摘    要:兰科(Orchidaceae) 植物俗称兰花,全世界约有700属20 000多种及大量变种,依其所属生态类型可分为地生兰、附生兰和腐生兰3类,作为一种重要的观赏花卉和药用植物资源,在花卉和天然药物产业上有着巨大的经济价值和利用潜力(罗毅波等, 2003).

关 键 词:兰属植物  菌根真菌  Rdna  ITS分析  瘤菌根菌  胶膜菌属
文章编号:1001-7488(2008)02-0160-05
修稿时间:2007年4月25日

rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants
Li Lubin,Hu Tao,Tang Zheng,Zhuang Caiyun,Liu Zhenjing,Yang Kai,Peng Zhenhua.rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants[J].Scientia Silvae Sinicae,2008,44(2):160-164.
Authors:Li Lubin  Hu Tao  Tang Zheng  Zhuang Caiyun  Liu Zhenjing  Yang Kai  Peng Zhenhua
Abstract:rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and belonged to different ecologic types.The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella,which was fully consistent with identification as Epulorhiza with morphological method.Additionally,based on rDNA ITS sequence cluster analysis,dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids(Cymbidium)symbiotic mycorrhizae and hosts.
Keywords:Cymbidium  mycorrhizal-fungi  rDNA internal transcribed spacer analysis  Epulorhiza  Tulasnella
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