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| 雄性不育烟草atp6基因育性相关生物信息学分析 | |
| 引用本文： | 周玮,刘齐元,朱腾义,范东东,朱肖文,程元强.雄性不育烟草atp6基因育性相关生物信息学分析[J].核农学报,2012,26(1):54-59,67. |
| 作者姓名： | 周玮 刘齐元 朱腾义 范东东 朱肖文 程元强 |
| 作者单位： | 1. 湖南农业大学生物安全科学技术学院／植物病虫害生物学与防控湖南省重点实验室,湖南长沙,410128 2. 江西农业大学农学院／作物生理生态与遗传育种教育部重点实验室,江西省作物生理生态与遗传育种重点实验室,江西南昌330045 3. 江西农业大学农学院／作物生理生态与遗传育种教育部重点实验室,江西省作物生理生态与遗传育种重点实验室,江西南昌330045／东南大学市政工程系,江苏南京210096 |
| 基金项目： | 国家自然科学基金项目(30660099),高等学校博士学科点专项科研基金新教师类资助课题(20114320120005) |
| 摘 要： | 线粒体基因atp6与多种植物细胞质雄性不育(CMS)关系密切。为探索atp6基因致烟草CMS的潜能,以2对烟草不育系和相应保持系为材料克隆atp6基因,测序结果显示不育系与保持系atp6基因间有6个碱基差异。进一步的生物信息学分析结果表明,6个碱基差异导致4个预测氨基酸残基差异及差异残基所处区域的亲/疏水性改变,不育系atp6基因预测蛋白在二级结构和结构域上均呈现出周期结构(主要为α-螺旋)增加而非周期结构(主要为无规卷曲)减少的趋势,不育系atp6基因的碱基差异能在一定程度上引起编码亚基空间结构类型发生改变。推测atp6编码亚基尤其是亚基结构域空间结构类型的改变可能干扰线粒体F0F1-ATP合成酶的功能,从而成为影响烟草育性的因素之一。 |
| 关 键 词： | 烟草 细胞质雄性不育 atp6基因 育性 生物信息学 |
| 收稿时间： | 2011-04-19 |
| 修稿时间： | 2011-09-13 |
BIOINFORMATICS ANALYSIS OF apt6 GENE RELATED TO TOBACCO MALE STERILITY |
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| ZHOU Wei,LIU Qi-yuan,ZHU Teng-yi,FAN Dong-dong,ZHU Xiao-wen,CHENG Yuan-qiang.BIOINFORMATICS ANALYSIS OF apt6 GENE RELATED TO TOBACCO MALE STERILITY[J].Acta Agriculturae Nucleatae Sinica,2012,26(1):54-59,67. | |
| Authors: | ZHOU Wei LIU Qi-yuan ZHU Teng-yi FAN Dong-dong ZHU Xiao-wen CHENG Yuan-qiang |
| Institution: | 1(1.Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy,Jiangxi Agricultural University,Nanchang,Jiangxi 330045;2.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,College of Bio-safety Science & Technology,Hunan Agricultural University,Changsha,Hunan 410128;3.Department of Municipal Engineering,Southeast University,Nanjing,Jiangsu 210096) |
| Abstract: | Mitochondrial gene atp6 had close relation with cytoplasmic male sterility (CMS) of various plants. To understand the potential function of apt6 gene on tobacco (Nicotiana tabacum) CMS, we cloned atp6 gene from two CMS lines and their maintainer lines. Sequencing results showed six different bases in atp6 genes for two lines. Bioinformatics analysis of their predicted protein revealed that the six base differences would lead to the differences of four predicted residues and the hydrophilic/hydrophobic profiles in corresponding regions. The predicted proteins from CMS lines presented a changing trend of increasing periodic structure (mainly being α-helix) but decreasing aperiodic structure (mainly being coil) in the secondary structures and domains. The above information indicated that the atp6 base differences of CMS lines could lead to the change in spatial structure of their coding subunits. It is presumed that structural differences in subunit 6, especially in its domain might be a potential factor that disturbed the function of F0F1-ATP synthetase in mitochondrion and thus became one of the possible causes of tobacco CMS. |
| Keywords: | tobacco cytoplasmic male sterility " target="_blank">atp6 genezz')" href="#"> atp6 gene fertility bioinformatics |
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