| 小球藻的分离及其DNA提取方法的研究(英文) |
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| 作者单位： | 石河子大学生命科学学院 |
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| 摘 要： | Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
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Isolation of Chlorella vulgaris and Its DNA Extraction Methods |
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| Abstract: | Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources. |
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| Keywords: | Chlorella vulgaris Isolation method DNA extraction method |
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