| 不同动物源性大肠杆菌多重耐药调控基因rob的同源性分析 |
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| 引用本文： | 马红霞,刘玉堂,伞治豪.不同动物源性大肠杆菌多重耐药调控基因rob的同源性分析[J].中国兽医科技,2007,37(12):1035-1040. |
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| 作者姓名： | 马红霞 刘玉堂 伞治豪 |
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| 作者单位： | 吉林农业大学动物科学技术学院,吉林长春130118 |
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| 基金项目： | 国家自然科学基金项目（30400326）;吉林省杰出青年基金项目（20050124）;吉林省科技厅项目（20030425-03） |
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| 摘 要： | 为研究大肠杆菌多重耐药调控基因rob与不同动物源性及多重耐药水平之间的关系，选取临床分离的不同动物源性大肠杆菌5株及大肠杆菌药敏质控株ATCC25922，在对其进行主要治疗药物耐药性检测的基础上，分别以其染色体DNA为模板，通过PCR反应扩增出大肠杆菌多重耐药调控基因rob，将该基因分别与克隆载体pMD18-T连接，连接产物转化至大肠杆菌JM109感受态细胞中，对经酶切与PCR反应鉴定为阳性的克隆质粒进行了核苷酸序列测定。测序结果表明：不同动物源性大肠杆菌的rob基因与Gen-Bank中该基因的核苷酸序列及所推导的氨基酸序列的同源性较高。不同源性大肠杆菌的rob基因的核苷酸序列与其动物源性有关，该基因的核苷酸序列的个别突变位点可能影响AcrAB外输泵的表达水平，进而影响其多重耐药水平。
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| 关 键 词： | 大肠杆菌 多重耐药 rob基因 克隆 同源性分析 |
| 文章编号： | 1673-4696（2007）12-1035-06 |
| 收稿时间： | 2007-07-26 |
| 修稿时间： | 2007-09-17 |
Homology analysis of multidrug-resistance regulating gene rob of Escherichia coli from different animal species |
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| MA Hong-xia,LIU Yu-tang,SAN Zhi-hao.Homology analysis of multidrug-resistance regulating gene rob of Escherichia coli from different animal species[J].Chinese Journal of Veterinary Science and Technology,2007,37(12):1035-1040. |
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| Authors: | MA Hong-xia LIU Yu-tang SAN Zhi-hao |
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| Abstract: | In order to study the relationship between multidrug-resistance regulating genes rob of Escherichia coli from different animal species and levels of multidrug-resistance,5 strains of E. coli isolated from different animal species and control strain ATCC25922 were selected. Based upon drug-resistance detection of main therapeutic drugs,rob genes of multidrug-resistance of E. coli were amplified by PCR from chromosome DNA of E. coli and ligated into pMD18-T Simple Vector. The ligated products were translated into E. coli JM109. A positive plasmid identified by enzymatic digestion and PCR was sequenced. The sequencing result indicated that homology of nucleotide sequences of and deduced amino acids from the rob genes between the 5 E. coli isolates and that of GenBank were high. Some gene mutations of rob gene of the 5 E. coli isolates were related to animal species and to the level of drug resistance by affecting on the expression level of AcrAB efflux pump. |
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| Keywords: | Escherichia coli multidrug-resistance rob gene cloning homology analysis |
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