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铀矿浸出液胁迫对绿豆种子萌发和幼苗生长及其抗氧化酶活性的影响
引用本文:聂小琴,丁德馨,李广悦,吴彦琼,刘玉龙,胡劲松,王友团.铀矿浸出液胁迫对绿豆种子萌发和幼苗生长及其抗氧化酶活性的影响[J].农业环境保护,2009(4):789-795.
作者姓名:聂小琴  丁德馨  李广悦  吴彦琼  刘玉龙  胡劲松  王友团
作者单位:南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001
摘    要:采用培养皿光照培养箱培养方法,研究了不同浓度铀矿浸出液胁迫对绿豆种子的萌发、幼苗及其根系的早期生长以及体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响。结果表明,铀矿浸出液的浓度≤10%时,胁迫对绿豆种子的萌发率没有影响,但加快了种子的萌发势,显著激发了绿豆幼苗及其根系的生长(P〈0.05);浓度〈10%时,铀矿浸出液胁迫显著增加了绿豆幼苗及其根系SOD的活性(P〈0.05),但它们的CAT活性接近对照;浓度=10%时,铀矿浸出液胁迫显著增加了幼苗及其根系CAT的活性(P〈0.05);浓度=100%时,铀矿浸出液胁迫显著抑制了种子的萌发势(P〈0.05),最终的萌发率只能达到60%,幼苗及其根系不能正常堆长.它们的SOD和CAT活怀远远低于对照。

关 键 词:绿豆(Phaseolus  vulgais  L.)  铀矿浸出液  种子萌发  幼苗生长  抗氧化酶

Effects of the Stress from Uranium Pregnant Solution on Seed Germination and Seedling's Growth and An- tioxidant Enzymes Activities of Mung Bean
NIE Xiao-qin,DIND De-xin,LI Guang-yue,WU Yan-qiong,LIU Yu-long,HU Jin-song,WANG You-tuan.Effects of the Stress from Uranium Pregnant Solution on Seed Germination and Seedling's Growth and An- tioxidant Enzymes Activities of Mung Bean[J].Agro-Environmental Protection,2009(4):789-795.
Authors:NIE Xiao-qin  DIND De-xin  LI Guang-yue  WU Yan-qiong  LIU Yu-long  HU Jin-song  WANG You-tuan
Institution:(Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China)
Abstract:Mung bean is one of the key edible beans in China. It is very important to prevent it from being polluted by radioactive nuclides. In this paper, the uranium pregnant solution from leaching of uranium ore was used to study the effects of the stress from the solution on the seed germination, the early growth of the seedling and its root and their superoxide dismutase and catalase activities. The research results showed that, when the proportion of the uranium pregnant solution in its dilution was less than or equal to 10%, the dilution would promote the seed's germination potential but would not affect its germination rate, and would stimulate the growth of the seedling and its root significantly. When the proportion was less than 10%, the dilution would activate the superoxide dismutase of the seedling and its root obviously, but would have little effect on their catalase activity. When the proportion was 10%, the dilution would activate the catalase activity of seedling and its root; And that, when the proportion was 100%, the seed's germination potential would be inhibited significantly, the final germination rate could only reach 60%, seedling and its root could not grow as normal and their superoxide dismutase and catalase activities would be much lower than those of seedling and root cultivated in distilled water.for comparison.
Keywords:mung bean  uranium pregnant solution  seed germination  seedling's growth  antioxidant enzyme
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